水通道蛋白在干燥綜合征小鼠頜下腺中表達(dá)及意義的實(shí)驗(yàn)研究.pdf

1、第三軍醫(yī)大學(xué)碩士學(xué)位論文水通道蛋白在干燥綜合征小鼠頜下腺中表達(dá)及意義的實(shí)驗(yàn)研究姓名：肖林申請學(xué)位級(jí)別：碩士專業(yè)：口腔頜面外科學(xué)指導(dǎo)教師：楊軍20030501第三軍醫(yī)大學(xué)碩士學(xué)位論文AbnormalExpressionofWaterChannelPnro。t，i—en，s，inPloj‘、SubmandibularGlandsfromSj／igren’SSyndromeMouseAbstractBackgroud：Sj69ren’SSyn

2、drome(SS)isacommoninflammatorydiseasecharacterizedbylymphocyticinfiltrationofsalivaryandlacrimalglandsleadingtoglaadularhypofunctionanddrymouthandeyessalivaryglandbiopsiesshowlymphocyticinfiltrateswithamaximal50％decrease

3、inacininumber，andMetalloprotenaseshavebeenimplicatedinacinardestructionHoweverinadditiontolossofglandelements，glanddysfuactionplaysanimportantroleintheclinicalexpressionofthediseaseSeveralfactorsmaycontributetoimpairedsa

4、livaryflowincludingproinflammatorycytokines，suchasIL1andTNF—athatcaninhibitbothbasalandstimulatedsecretionDecreasedproteinkinaseClevelsinsalivaryglandacilarcellsofSSPatients，alongwiththepresenceofantibodiesagainstmuscari

5、nicM3receptorinprimarySSpatientscouldpreventnormalglandresponsetoneuronalsimulation，Inanormalsalivarygland，theacinisecreteaprimaryisotonicfluidfromwhichconsiderableNaCtisresorbedduringthepassagethroughtheductsDuctalcellp

6、ossessasodiumchannel，achloridechannel，andasodium—protonexchanger，whicharelikelytobeinvolvedinNaCIreabsorptionRecentdiscoveryofafamilyofwatersyecificmembranechannelproteins，theaquaporins，providedinsightintothemolecularmec

7、hanismofmembraneWaterpermeabilityinavarietyoftissues，AQPprovidesthemolecularpathewayforwaterreasorptionintherenalproximaltubuleandvasarecta，participatesinlungwaterhomeostasis，andinthesecretionofcerebrospinalfluidandbile，

8、ItWasalsoconfirmedtodistributinginsalivaryglandtissues；AQP5wasclonedfrcmratsubmandbularglandsandispresentinthelacrimalglandandlung，suggestingrotesingenerationofsalivatearsandpulmonarysecretionsAmongtheaquaporinsidentifed

9、todate，onlyAQPt，AQP2andAQPshavebeenimmunohistochemicallylocalizedtohumansalivaryglandsAbjective：Todevelopeamousemodelfornon—bacterialsialoadenitissimilartoSj69ren’SSyn&omeandexaminethedistributionofvarioussubtypesofwater