酪氨酸可逆蛋白磷酸化在植物細(xì)胞逆境信息傳遞中的作用.pdf

1、中國(guó)農(nóng)業(yè)大學(xué)博士學(xué)位論文酪氨酸可逆蛋白磷酸化在植物細(xì)胞逆境信息傳遞中的作用姓名：魏開(kāi)發(fā)申請(qǐng)學(xué)位級(jí)別：博士專(zhuān)業(yè)：果樹(shù)學(xué)指導(dǎo)教師：賈文鎖胡建芳20070601Abstractplantgrowthanddevelopmentarcinfluencedordisruptedinevitablybyenvironmentalstresses，plantshaveobtainedthemechanismandstrategiestoadaptor

2、resistantagainstexternalenvironmentalconditionsduringlongtimeevolutionCellsignalingtransductioniscriticalforsress_resistanccinPlantsandStresssignalingtransductionpathwayisverycomplexTheabscisicacid(ABA)’akeysignalingeomp

3、onent，regulationoftheoriginofABAsignalingplayimportantroleinstresssignalingpathwaysThisresearcholltheregulationmechanismofNCED3andAd03inABAbiosynthesisindicatereversibleproteintyrosinephosphorylationinvolvedinoriginofABA

4、signalingOurresearchhasfoundthatthekeyABAbiosynthesisgenesNCED3and4403expressedspeciallyinvascularbundletissuefurtherexperimentofsubcellularlocalizationthatNCED3andAA03localizedspecificlyinchloroplastandcytoplasmcorrespo

5、ndinglyshowthatthetransmembranetransportofprecursormalghtmanipulatetheoriginofABAsignalingAsNCED3andAA03couldbeinducedsignificantlybydroughtstress，WiththehelpoftransgeniesystemoftightlycontrolledandhigIllyinducibleXVEexp

6、ressionsystem，increasingNCED3expressionupregulateAd03transcriptionallevel，theseobservationssuggestNCED3isinvolvedinapositivefeedbackregulationofAA03geneexpressionunderdroughtstressWefmdadublefunctionalkinaseofMAPKKKfamil

7、ynamedasMAPKKK6(42)inlargescaleArabidopsismutantscreeningThemutantofMAPKKK6(42)leadstolowertranscriptionallevelof4A03studiesshowthatitlocalizedspecificlyinthemembranechemicalinduciblesystemsidentifiedthatincreasingexpres

8、sionofM4egrac6(42)upregnlatetheexpressionofAdOJgenethefindingssugestthatMAPKKK6(42)mightplayimportantrolesintheregulationofABAsignalingaccumulationExperimentsofpharmacologyindicatethatPTPaSehavearelationshipwithoriginofA

9、BAsignalingassp∞incal|yinhibitorofPTPasccanefficientlyinhibitedABAaccumulationZmRIDPIamemberof]rrp目kqefamilywasfirstclonedandputtiedfrommaizebourlab，theexpressionofZmRIDPIupregulatcobviouslytheproductionofAA03Mmatheresul

10、tmeansP1ma∞involvedintheABAsignalaccumulationAntotherPTPasewasfoundinArabidopsisnamedasPTPl35themntuant醴PTPl35leadstolowertranscriptionalevelofdroughtinduciblegeneLTl30TheganeexpressionanalysisshowedthatinoverexpressionP

11、TPl35plants，upregnlatetheexpressionofTUBlmRNA，downregulatetheexpressionofCOFTCHSREDl。PIP4。GI。HRBIPIP3CR88ELF3，havelittleinfluence∞RBC$M，工觀CCAIWeidentifiedthatP1Pa∞notonlytakepartinthestressesmessengertransportbutalsoinvo