新1，4-二氧喹喔啉衍生物的合成、抑菌活性及與血清白蛋白相互作用的研究.pdf

1、新疆農(nóng)業(yè)大學(xué)碩士學(xué)位論文新1，4二氧喹喔啉衍生物的合成、抑菌活性及與血清白蛋白相互作用的研究姓名：孔新剛申請學(xué)位級(jí)別：碩士專業(yè)：動(dòng)物營養(yǎng)與飼料學(xué)指導(dǎo)教師：李華20080601AbstractAccordingtolittleadversereactionandmorebioactivityofthederivativesofquinoxaline14dioxide，thethesishassynthesizedthreenewderiv

2、ativesofquinoxalinel，4dioxide，andhadbeencharacterizedbymeansofelementalanalyses，MS，IRand1HNMlLTheantibacterialactivityexperimentofCI～CⅢwascarriedbydiskdiffusionmethodandminimuminhibitoryconcentration(MIC)methodtostudythe

3、antibacterialactivityAndtheinteractionbetweenCI～CⅢandbovineserumalbumin∞SA)WasstudiedbymeansofspectraonthebasisofthebindingsiteofbiologybigmoleculeandmedicinesmallmoleculeinteractionUseonitroanilineasrawmaterial，andaquir

4、ethetargetderivativesofquinoxaline1，4dioxide(cI～cllI)thoughNaCIOoxidizingreactionBeirutreactionSe02oxidizingreactionandcondensationreactionWeimprovedSe02oxidizingreactiononthebasisofpredecessor，whichuse14dioxaneasresolut

5、ionaddrequiredamountofSe02atonetime，took5hoursinthe100“(2oilbathAndtheintermediateproduct(2ethylformate3formaldehydequinoxaline1，4dioxide)WasnotnecessarytObeenpurifiedwasdirectlymadeUseofcondensationreactiontotalyieldwas

6、76％TheantibacterialresultsshowedthatCI～CⅢhadstronginhibitOryactivityagainstsixtrialedbacteria(ieEscherichiacoli、Bacillussubtilis、Staphlococcus_f2ureus、Bacillusmegaterium、Sporosarcinaurea、Bacillusthuringiensis)butnoinhibi

7、toryactivityagainstfungl(Penicillium、Phizopussp、Aspergillusniger)WasobservedMICofCItoEscherichiacoliis20pg‘mL“。andtoBacillusmegateriumis15pg‘mL“1；MICofCIItoEscherichiacoliislOpgmL“1andtoStaphlococcusaureusis15pgmL“1：MICo

8、fCmtoEscherichiacoliis151上g‘mL“1andtoBacillusthuringiensisis201LgmL“‘TheinteractionbetweenQuinoxalineI，4dioxide(CICITI)andbovineserumalbumin(BSA)WasstudiedbyfluorescencespccllodandultravioletspectraThebindingconstantKand

9、thebindingsitesnwerefoundbyfluorescencequenchingmethodThebindingdistancerandenergylransferefficiencyEbetweenCI一CmandBSAwcrealsoobtainedaccordingtotheF6rstertypodipoledipolenonradiativeeneq；ytransfermechanismTheeffectofCI

10、“。CIlIontheconformationofBSAWasalsoanalyzedbyusingsynchronousfluorescencespectroscopyInadditional，theimpactofCI～CⅢonconformationofBSAWasexaminedbysynchronizedspectrum，thehydrophobicinteractionbetweenCI一CmandBSAisstrongan

11、dCIClllCaneffecttheconformationofBSAinsomedegreeAccordingtoitwewillobtainthatCI～CIIImayreachtecipientandtakeanactionbystoringandcarryingofbloodplasmKeywords：Quinoxaline1，4dioxide，Synthesis，AntibacterialActivity，SerumAlbu