瓊膠酶產(chǎn)生菌株的選育及酶解條件和酶解產(chǎn)物的研究.pdf

1、青島海洋大學(xué)碩士學(xué)位論文瓊膠酶產(chǎn)生菌株的選育及酶解條件和酶解產(chǎn)物的研究姓名：王靜雪申請學(xué)位級別：碩士專業(yè)：水產(chǎn)品加工與貯藏指導(dǎo)教師：江曉路2001.6.1ABSTRACTWiththepurposeoftheselectingofastrainwithpreferredpropertiesproducingagaraseandpreparingtheoligosaccharideswithdifferentdegreeofpolymer

2、izationbyenzymaticmethod，weresearchtheeffectsofdifferentfactorsonfermentationandproducingagarasebystrainandthebasicpropertiesoftheenzyme；wealsoanalyzethedifferent01(1譫)T08he∞s曲tra刪in郎tha㈣tco“uld呵pr幽oduc鯽e：黧wasselectedfro

3、malgaeWe鯧ara菇。enhancetheabilityofproducingagarasebyultravioletmutationandchemicalmutation(2)Withthesinglefactortestandrelationshiptest，wedecidedanoptimumculturemedia(agarO2％，K2HP0401％，triAmmoniumcitrate01％，NaCl1％)andanop

4、timumcultureconditions(125mlliquidculturemediaWasin250mlErlenmeyerflask；culturetemperature，pH，rotationrateandculturetimewere30“(2，pU65，150rpmand26hrespectively)(3)TheenzymeWaSseparatedby80％ammoniumsulfatefractionationThe

5、baSicpropertiesoftheenzymewerestudiedaSfollows：TheoptimumpH，theoptimumtemperatureandtheoptimumNaCIconcentrationwerepH7035“Cand2％respectivelyTheenzymeWaSactivatedbyK十、Ca2、Na、Fe3、Fe2andinhabitedbyM92、Mn2(4)Theproductofdegr

6、adationwereseparatedintoseveralsamplebyethanolgraduallyIonexchangechromatographydividedandpurifiedthethreesamplethathadtheleaStdegreeofpolymerizationtoachievedthreemainblocksThemolecularmasswereaScertainedbyMSThestudiesp