豬皮酸解和酶解產(chǎn)物的制備及其抗氧化活性的研究

1、中國農(nóng)業(yè)大學(xué)碩士學(xué)位論文豬皮酸解和酶解產(chǎn)物的制備及其抗氧化活性的研究姓名：陳霞申請學(xué)位級別：碩士專業(yè)：農(nóng)產(chǎn)品加工與貯藏工程指導(dǎo)教師：戴瑞彤馬長偉20070601AbstractPigskinwithhi曲contentofnaturalcollagenwasusedasrawmaterialofthisstudy1nbehydrolysisofthecollagenwasobtainedbyHC!treatment∞dpepsindig

2、estionInordertofindtheoptimumhydrolysisconditionFerricthiocyanatemethod(FrC)aIldT11iolm“turicaxedreactive目abstanceaassay(TBAPS)weleusedtoestimatetheantioxidativeactivityofthehydrolysatesThenatulalcollagenhasstablestructu

3、reandisinsolubleinwateranddiluteacidSobeforehydrolysis，thepigskincollagenWasdenaturalizedwithhightemperature121“(2toincmasehydrolysisefficiencyBecauseofthelowmolecularweightoftheacidhydrolysate，containedhighlevelofsaltSe

4、phadexG25wasusedtodesaltforthehydrolysate，anddesalinationeffectWasobservedbymeasuringelectricconductivityTheeffectsofthedenaturalizationtimeandacidhydrolysistimeⅥmstudiedduringtheacidhdrolysis∞中口im仳Themsultshowed：thehydr

5、ochloricacidconcentrationO5mol／Lthedenaturalizationtime5rainthehydrolysistime7hInthisconditionthehydrolysatehadhighentioxidafiveactivityTheeffectsofthedenaturalizationtime，enzymedosageandenzymehydrolysistimeWmstudiedduri

6、ngtheenzymehdrolysisexperimentTheresultshowed：thedenaturalizationtime5miIl’aceticacidconcentration05mot亂enzymedosage(enzyme：pigskin)15％，thehydrolysistime05hBoththeacidhydrolydsteandenzymehydrolydstecouldreUraintheoxidati

7、onofthelardbutfileantioxidafiveactivityofacidhydrolydstewashigherthanthatoftheenzymehydrolydsteBoththeanfioxidafiveactivitiesoftheacidandnI刁fH”hydrolydsteswerelowerthanthatoftheTBHQSephadexG25(Medium)wasusedtodesaltforth

8、eacidhydrolysateTheresultshowedthattheflowrate$vasthemainfactorforthedesalinationeffectn垃optimumconditonweresamplevolumetml，detectionwavelength220hmtheflowrate45ml／tOminandthesampleconcentration029／m1Key：collagenofpigski