氧化硅介孔泡沫材料的制備及其木瓜蛋白酶固定化.pdf

1、北京工業(yè)大學碩士學位論文氧化硅介孔泡沫材料的制備及其木瓜蛋白酶固定化姓名：鄒澤昌申請學位級別：碩士專業(yè)：材料學指導教師：韋奇20090501ABSTRACTABSTRACTSiliceousmesocellularfoam(MCF)，anewmaterial謝t11continuous3Dporestructureconnectedbywindowthelargestporesizecomparedtoothermesoporousma

2、terialsandhi曲hydrothermalstabilitymaybeoneofthemostpromisingsupponSforenzymeimmobilizationAsanenvironmentlyfriendlybiocatalystimmobilizedenzymecancatalyzesomechemicalreactionshighlyselectivelyandefficientlyundergentlerea

3、ctionconditionsThisdissertationfocusesonthepreparationandstmcturecharacterizationofMCFmaterials，theimmobilizationofpapainonMCFandthepropertiesandthestabilitiesofimmobilizedpapainSiliceousmesocellularfoam研tllporesizerangi

4、ngfrom124to301nlTl，andwindowsizefrom32to185nnl，wassuccessfullysynthesizedbytheacidcatalyzedsolgelreactionoftetraethoxysilane(TEOS)inthepresenceoftriblockcopolymerP123asastmcturedirectingagent，trimethylbenzene(TMB)asaswel

5、lingagentandammoniumfluoride(NI14F)asawindowsizeregulator111eaminofunctionalizedMCF(NH2MCF)，preparedviapostgraRingmethod誦maminopropyltrimethoxyl—silane(APTMS)assilaneadditive，hadbeendemonstratedtopossessadesirablemesopor

6、ousmucnlrebymeansofXRD，TEM，F(xiàn)TIRandN2adsorptionBothMCFandNH2MCFmaterialwereemployedascarriersforpapalnimmobilizationThepropertiesofimmobilizedenzymewerecloselyrelatedtothewindowsizeofMCEAwindowsizenearlyequaltothediameter

7、ofenzymeledtothehighestadsorbedamountandactivitiesofimmobilizedenzymeComparedtounmodifledMCFmaterials，theaninlo—functionalizedMCFwasmoreefficientintheenzymeimmobilizationItWasshownthattheexperimentalconditionsforenzymeim

8、mobilizationaffectedthepropertiesandthestabilitiesofimmobilizedpapin111eoptimalconditionsofpapainimmobilizationcouldbesummarizedasfollows：papainconcentration，067mg／ml；pH，70；temperature，37℃andimmobilizationtime，6hTheoptim

9、alpHandreactiontemperatureoftheimmobilizedpapainpreparedundertheaboveoptimalconditionswere75and50℃。comparedto70and40℃forthefreepapainrespectivelyTheMichaelisconstant(點鋤)ofimmobilizedpapainwasdisclosedas699x10dmol／LbytheL

10、ineweaverBurkplotat37℃Afterstoredat4℃for60days，theimmobilizedpapainretained75％ofitsactivitycomparedto536％forthefreepapainAbout651％oftheimmobilizedpapainactivityremainedafterrepeatingthecatalysisofcaseinfor8timesDuetothel