褐藻多酚化合物提取、純化及生物活性研究.pdf

1、青島大學(xué)碩士學(xué)位論文褐藻多酚化合物提取、純化及生物活化研究姓名：于曙光申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：應(yīng)用化學(xué)指導(dǎo)教師：魏玉西2003.5.20AbstractSargassumKjellamanianumYendoandSargassumthunbeergiikuntzearetwocommonkindsofbrownalgaealongQingdaocoastTheyareabundantinbioactiveconstituents，wh

2、ichmakethempotentialresourcesforadvancedpharmaceuticalutilizationThepresentthesisisfocusedOllthestudiesoftheextraction，purificationandbioactivitiesofthephlorotanninsinthetwobrownalgaeTheresultsoftheexperimentsareasfollow

3、s：1TheConditionswechoosefortheextractionofthephlorotannins：Solventis90％alcohol；Temperatureis30℃：Thedurationis12handtheliquidnitrogenisusedbeforegrounded2Thebioactivitiesofthecrudeextractofbrownalgaephlorotanninsincludean

4、tioxidation，antibiosis，anticoagulation，andSOon(1)ThecrudeextracthasstrongactivityofscavengingfreeradicalsAsmarinenaturalproducts，theyhavesignificantadvantagecomparedwithsynthesticantioxidant(2)Thecrudeextracthasantibacte

5、rialactivitiesonbothpositiveandnegativebacteria，especiallyontwokindsofmarinebacteria(3)ThecrudeextracthasevidentanticoagulationactivitiesInvitro，theYcansharplyextendplasmacalciumrestorationandcoagulationtimeInvivo，theyca

6、nchangethetimeandthequantitiesofbleeding，thelengthofthrombi，thequantitiesandaggregationrateofbloodplates，andSOon3Thepurificationoftheextractcrudecanbedividedinto11bioactivefractionsThemethodsusedinthisprocessincludeTLC，c

7、olumnchroma￡ographyonsilica，sephadexGandsephadexLH一20，superfiltration4Thebioactivitiesofthe11fractionsarestudied，andrelatedtotheirmolecularweightsaccordingtotheexperimentalresultsThebiggerthemoleculeweight，thestrongerthe