硫酸軟骨素對HL60細(xì)胞增殖和分化的影響及應(yīng)用.pdf

1、青島大學(xué)碩士學(xué)位論文硫酸軟骨素對HL60細(xì)胞增殖和分化的影響及應(yīng)用姓名：虞浩申請學(xué)位級別：碩士專業(yè)：病理生理學(xué)指導(dǎo)教師：耿芳宋崔瑞耀20050528Chondroitinsulfate’sinfluencetotheproliferationandthedifferentiationofHL60cellandit’SproappliedstudyAbstractpurposeObservingtheinfluenceofChStothe

2、proliferationandthedifferentiationofHL60cell，inquiringtheChS’SapplicationvalueinchemotherapyofmalignanttumorMethodHL60cellswithsamedensitywasdividedinto5groupsatrandom，addedwith0“g／ml，5pg／ml，25pg／ml，50“g／mland75“g／mlChS①

3、Hatchingfor24hours，proceedcellcountandcomparethedifferentclonegrowthratewitheachothergroups②AddADRtoeachgroupsaRerhatchingfor24hours，thenhatchingfor12hourssequentiallycomparethedifferentsurvivingrateofcellwitheachothergr

4、oups③Hatchingfor5days，measurethecell’SNBTdeoxidizationabilitycomparethedifferencewitheachothergroups@Hatchingfor5days，addinktoeachofthegroups，observeandcomparethedifferentgobblingabilityofcellwitheachothergroupsbyhighsca

5、lemicroscope蓮)Hatchingfor5days，measuretheacidphosph&ruseactivitycomparethedifferentWiIheachothergroupsResult1AlltheconcentrationofChScanincreasetheclone—growthrateofcellobviously(P005)2AfterADRhadbeenadded，thecell’Ssurvi

6、vingrateofeachChSgroupsshownadecreaseindifferentdegree，especiallytheChSconcentrationabove25tIg／ml，3NBTdeoxidizationabilityandacidphosphataseactivityofeachChSgroupsboostedupobviously(P005)，alltheChSgroupsdisplayedastrongg

7、obblingabilityConclusion1ChScanaccelerateHL60cell’Sproliferation；2ChScanincreasethesensibilityofHL60celltoADRandboostobviouslyupthekilleffectofADRtoHL60cell；3ChScanpromotethedifferemiationofHL60cellPostgraduate：YuHao(pat