肌層浸潤性膀胱癌的間質(zhì)蛋白表達(dá)譜研究.pdf

1、青島大學(xué)碩士學(xué)位論文肌層浸潤性膀胱癌的間質(zhì)蛋白表達(dá)譜研究姓名：高偉申請學(xué)位級別：碩士專業(yè)：外科學(xué)（泌尿外科）指導(dǎo)教師：陳強(qiáng)20120602嘲ResearchonstromalproteomeexpressionprofileandmuscleinvasivebladdercancerAbstractObjectives：Togloballycharacterizethecancerstromaexpressionprofileofmus

2、cleinvasivebladdertransitionalcellcarcinomaandtOdiscussthecancerbiologyaswellasbiomarkerdiscoveryfromstromaMethods：Highlypurifymuscleinvasivebladdertransitionalcellcarcinomaandnormalstromabylasercapturemicrodissection(LC

3、M)；Separationofpeptidemixtttrebytwo—dimensionalliquidchromatography(2D—LC)followedbyelectrosprayionizationtandemmassspectrometry(ESIMS／MS)identification；BioinformaticsanalysiSoftheidentifiedproteins；GObiologicalprocessan

4、alysis；Pathwayanalysisindifferentlyexpressedproteins；EvidencebasedbiomarkerexplorationResults：Weidentified868／872commonlyexpressedproteinsand978differentialproteinsfrom4pairedcancerandnormalstromalsamples487／491proteinss

5、pecificexpressedincancer／normalstromaDifferentialproteinswerecomparedwiththeentirelistoftheintemationalproteinindex(IPI)，andtherewere42／42geneontology(GO)termsexhibitedasenrichedand8／5exhibitedasdepletedincellularcompone

6、nt，respectivelySignificantlyalteredpathwaysbetweencancer／normalstromamainlyincludemetabolicpathways，ribosome，focaladhesionetcFinally，descriptivestatisticsshowthatthestromalproteinswithextremesofPIandMWhavethesameprobabil

7、itytobeabiomarkerConclusions：TheseobservationspromisenewbiomarkersofprognosisandanewcompartmenttotargettherapyFurthermore，itisnowwellacceptedthatthecanceranditsstromahaveabidirectionalrelationshipatmultiplelevelstoelicit

8、carcinogenesis，invasionandprogressionAsoneofthesolidtumorwecanimaginethatstromalcellsplaysacriticalroleinmuscle—invasiveBTCCbasedonsubstantialevidences，thoughtherehasbeenneportoflargescaleproteomicsstudyonthemuscle—invas