2023年全國碩士研究生考試考研英語一試題真題（含答案詳解+作文范文）_第1頁
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1、寧夏大學碩士學位論文楊樹ISSR指紋圖譜構建及應用研究姓名：肖哲麗申請學位級別：碩士專業(yè)：生物化學與分子生物學指導教師：李健201103IIAbstractPoplarisacommonnameofalltreespeciesingenusPopulusoffamilySalicaceaeisacterizedbyrapidgrowthhighadaptabilitywideemployment.Fmanyyearsmanyachiev

2、ementsinbasicscientificresearchofpoplarhavebeenobtainedinourcountry.Littleiscurrentlyknownaboutthemajityofpoplarspeciesoccurringinmoleculargeicsvariationsystematicevolution.Alongwithsuccessiveappearofnewvarietiessomecons

3、iderableconfusioncausedintaxonomyleadstofussinessofvarietalcomplexitygeicbackground.Thetraditionalmarkerscantsatisfytherequirementstoidentifythepoplarspeciesalotofdifficultyariseduringdevelopmentofgeicresourcestheapplica

4、tionofimprovedvarieties.Indertoutilizeconservepoplargeicresourcesreliablemethodshadbeendevelopedintheanalysisofplantgeicdiversityintheexplationoftheirgeicrelationship.Scientificfoundationcouldbeprovidedfgeicimprovinguseo

5、fpoplar.Therefetheresearchinpoplarbreedingscreeningoftheparentshassignificantimplication.TheresearchusingISSRDNAmolecularmarkerscombinewithmphologicalfeaturestoanalysepoplargeicdiversityconstructgeicfingerprintsof57sampl

6、es.Itcanprovidesolidfoundationfbetteruseofpoplargermplasmresources.Theresultsareasfellows:1.57sampleswerecollectedfromYinchuanBotanicalGardenmostthemareNthwestcommonpoplarvarieties.20samplesamongthemareknownspeciesfromLe

7、uc.Duby、AigeiroDuby、TurangeBgeTacamahacaSpach.2.ThebestamplificationsystemfISSRPCRhavebeenestablishedtothesematerialsthetotalvolumeis25μLincludingtemplateDNA40ngprimer0.4μmolLMg21.5mmolLdNTP0.25mmolLTaqDNAployase0.5Upure

8、water.Thereactionprogramwasdevisedf36cycleseachwith94denaturationf30s℃annealingf30s72extensionf1min94predenaturef3mininthefmerdenature72℃℃℃postextensionf5mininthefinalextensionfinallypreservationat4℃.3.NineISSRprimerswhi

9、chgeneratepolymphicclearbwereedfconstructingfingerprintinganalyinggeicdiversityofpoplaramong100primerswhichfirstwerepublishedbyColumbiaUniversity.4.ThemolecularmarkersofISSRwereusedtoanalyzethegeicdiversityconstructfinge

10、rprintof57poplarcultivarsamongfourpoplarfactions.TheresultsindicatethatthesamplesofLeuc.Dubyhavealargeproptionamongallcollectedpoplarspeciesgeicrelationshipismerelativelyregularclusterresultsareclearerbutthegeicrelations

11、hipofpoplarsamplesamongLeuc.Dubyaremecomplex.Therestofotherfactionswereclusteredtogetherearlyrespectively.Thisresultconfmswithtraditionalmethodaccdingtomphologicalacters.Thiswillbeofvaluetoverifyeachother.5.57poplarmater

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