色素親和色譜和流通色譜模型研究.pdf

1、⑧中國近代第一所大學FOUNDEDlN1895天洋大尊TIANJlNUNIVERSITY碩士學位論文●學科專業(yè)：作者姓名：指導教師：生物化工李威孫彥教授天津大學研究生院2005年1月ABSTRACTThemathematicalmodelingofchromatographyisofparticularinteresttobioengineers，sinceitcanbeusedasausefulguideandpowerfultool

2、intheoptimaldesignoftheoperationschemeorscaleupofachromatographyprocess“／hefollowingworkhasbeendoneabouttheoriesfordye—ligandaffinitychromatographyandflowthrou【曲chromatography：Amathematicalmodelfurdyeligandlineargradient

3、chromatographyofabinaryproteinsystemwasestablishedbasedontheaxialdispersiontheoryandporediffusionmodelBovineserumalbumin(BSA)andhemoglobin(Hb)wereusedasmodelproteinsandCibacronblue3GA(CB)modifiedSepharoseCL一6B(CB—Sepharo

4、se)wasusedasadsorbentThebinaryadsorptionisothermsoftheproteinswereexpressedbythestericmass。action(SMA)formulismItwasfoundthatthemodelpredictionsagreedreasonablywellwiththeexperimentaldataobtainedundervariousconditionsIti

5、ndicatesthatthebinarySMAformulismwasusefulforsimulationofnonlinearchromatographyAmathematicalmodelforflowthrough(perfhsion)chromatography，namelypacked—microtubes(PMT)model，hasbeenproposedforacolumnpackedwithbiporousanion

6、exchangerinwhichthemicroporesandflowthmughporesarecreatedwithliquidandsolidporogens，respectivelyThemodelisestablishedbasedOntheassumptionthatthebiporousparticleismadeupofpackedmicrotubesThreekindsofanionexchangers(ie，mic

7、roporous，macroporousandbiporousresins)arepreparedandwereusedasadsorbentstodeterminethemodelparametersandtoevaluatethemodelItisfoundthatthemodelpredictionsagreereasonablywellwiththeexperimentaldataobtainedundervariouscond