cDNA-AFLP技術分離和分析荔枝胚胎發(fā)育相關基因.pdf

1、福建農林大學碩士學位論文cDNAAFLP技術分離和分析荔枝胚胎發(fā)育相關基因姓名：譚全偉申請學位級別：碩士專業(yè)：植物學指導教師：陳偉王平20100401福建農林大學碩士學位論文ABSTRACTLitchiisoneoftheimportantsub—tropicalfruittreesEmbryodevelopmentstatusdoesnotonlydeterminethesizeofseedsanddirectlyaffecttheq

2、ualityoffruitanditsprocessingquality，butalsocloselyrelatedtolitchiproductionTherefore，thestudyofEmbryoabortionmechanismsnotonlytoprovidetheoreticalsupporttolitchibreeding，andhaspracticalvalueinproductionThisstudyaimsatfi

3、ndingtheinformationofgenesassociatedwithEmbryodevelopmentbyeDNA—AFLPandblotting，andofferreferencestotheresearchofmolecularmechanismofLitchiEmbryodevelopmentandtakingcontrolmeasuresinfutureInthisstudy，theeDNAofthreekindso

4、fmaterialswhichincludenormaltypeembryoes，partiallyabortiveembryoesandfullyabortiveembryoesWasanalyzedbyeDNA—AFLPMorethan60painofprimercombinationswereScreenedforconstructingEmbryoDevelopmenteDNAAFLPmapThroughtheanalysiso

5、fthemappolymorphism，60werepickedandrecoveredfrommorethan3000TDFsTheyweresequenced，homologyanalyzedandblottingverified31TDFsweresequencedSuccessfully7TDFswerefoundtohavehigherhomologywithEmbryodevelopmentrelatedgenesthrou

6、ghBLAST，whichwereTDFl(F3II)，TDF2(AP43A)，TDF3(CCR4NOT)，TDF26(GRP23)，TDF38(phospholipasegene)，TDF45(phosphatidylinositolkinasegene)，TDF55(caseinkinasegene)TwoTDFswererandomlyselectedtobeverificatedbyblottingTheresultswerec

7、onsistentwiththeelectrophoresisItisreportedthatthese7genesaremainlyrelatedtosignaltransductionandRNAinterferenceduringEmbryodevelopmentBlottingresultsshowedthatTDF1Wasdetectedin‘WuYe’and‘LanZhuTDF38onlyWasdetectedin‘LanZ

8、huwhichwereeonsigentwitheDNA—AFLPmapTherefore，itisspeculatedthatregulationofsignaltransductionandregulationofRNAinterferencepathwayexistinlitchiEmbryodevelopmentIfsuchtwokindsofpathwaysareabnomal，itwillleadtoembryomidway