水牛卵巢卵泡顆粒細(xì)胞凋亡的生物學(xué)特征.pdf

1、廣西大學(xué)碩士學(xué)位論文水牛卵巢卵泡顆粒細(xì)胞凋亡的生物學(xué)特征姓名：李恭賀申請學(xué)位級別：碩士專業(yè)：預(yù)防獸醫(yī)學(xué)指導(dǎo)教師：何寶祥20050501BiologicalcharacteristicsofbuffaloovarianfolliculargranulosacellsapoptosisAbstraetGenerally，themostoffolliclesundergoatresiaanddegenerationduringtheirdev

2、elopmentinmammals，andonlyafewofthemgrowunimerruptedlytomaturationundlovulationThemechanismofthefollicleatrisiastillremainsunclearalthoughmanystudiesonthefollicleatresiahavebeenconductedbymanyresearchersInthisstudysomeexp

3、erimentsonfollicularatresiawerecarriedoutinbuffaloasfollows：1、Themorphologicalchangesofapoptosisontissuesectionsofhealthyfolliclesandatreticfolliclesinbuffalo’Sovarieswereobservedbylightmicroscopeandtransmitelectronicmic

4、roscope，theapoptosisofthegralulosacellsdrewfrombuffaloovarianfolliclewasexaminedbyfluoromicroscopeTheresultshownthatfollicularcellshavetypicalchangesofapoptosissuchas，condensationofchromatin，nucleoplasmicsegmentationandc

5、ongregatedundernuclearmembrane；cellmembraneshrinkage，organdiecongregateandweredark—stainedundermicroscopeTherewereamaSsofapoptoticbodieswhichwereenwrappedbyintegratemembraneinatresicfollicularcavity；Apoptoticgranulosacel

6、lsdisplayedbrighterbluethannormalcellsunderfluoromieroscope2、The“Smear”patternthatreflectedDNAbreakdownwasnotfoundinhealthyfolliclesandthe“smear”wasfoundinatreticfolliclesbygranulosacellsDNAelectrophoreticanalysisButthet

7、ypical“l(fā)adder’’pattemofgranulosaapoptosiswasnotfoundinbothhealthyfolliclesandatreticfollicles3、Aoptoticchangesofgranulosacellswereexaminedinthehealthyandatretictollielesofbuffalo’SovariesbyTUNEL、theresultsshowedthatthere