大豆凝集素對大鼠胰腺生長及胰酶活性的影響.pdf

1、中國農(nóng)業(yè)大學(xué)博士學(xué)位論文大豆凝集素對大鼠胰腺生長及胰酶活性的影響姓名：臧建軍申請學(xué)位級別：博士專業(yè)：動物營養(yǎng)與飼料科學(xué)指導(dǎo)教師：李德發(fā)樸香淑20060601AbstractFivestudieswereconductedtoinvestigatethemechanismofsoybeanagglutininonpancreaticgrowthandtheactivitiesinratsInexpedment1，samplesofpure

2、soybeanagglutininwerepreparedbyaffinitychromatographyTheresultsshowedthatthecontentsandhemaglutininatingactivityofsoybeanagglutininsamples，whichwerepurifiedfromlawsoybeanseeds，wereapproximatetothoseofthestandardproductof

3、soybeanagglutininInexperiment2，30ratswereadministeredwith0，35，70，105，or140mgofsoybeanagglutinin(n=6)bygastricinfusiononceadayfor14daysComparedwiththecontrolgroup，soybeanagglutininreducedweightgain，enhancedabsoluteandrela

4、tivedrypancreaticweightandelevatedserumcholecystokininlevels妒005)Italsodepressedtherelativedrykidneysandspleenweights，andsystemicinsulinlevelsPancreaticprotein，RNAratherthanDNA，andpancreaticactivitiesofamylase，trypsinand

5、chymotrypsinallincreasedinresponsetoincreasedlevelsofsoybeanagglutinin(P005)Theresultssuggestedthatsoybeanagglutinininducedcholecystokininmediatedpancreatichypertrophyandpancreaticenzymesynthesis，andalsoinfluencedimmunea

6、ndmetabolismsystemsInexperiment3，duodenalcellsobtainedfromtworatsincubatedwitheithersaline，100Itg／mLsoybeanagglutinin，oramixtureof100llg／mLsoybeanagglutininand10mmol／LofverapamilSoybeanagglutininfP005)stimulatedcholecyst

7、okininmRNAexpressionandcholecystokininreleasefromsmallintestinalmucosalcells，howeverthoseeffectsWasabolishedbyverapamil，whichindicatedthatsoybeanagglutininstimulatedcholecystokiningeneexpressioninthesmallintestine，anditw

8、asrelatedtotheL—typecalciumchannelExperiment4，westudiedthepancreaticproteincontent，nucleicacidcompositions，pancreaticenzymeactivities，serumnitricoxidesynthaseactivities，andoxide／guanosinecyclicmonophosphate(NO／cGMP)level

9、responseineighteenmaleratsaftercontinuousadministrationofcholecystokininoctapeptideornitricoxidesynthaseinhibitoronceadayfor10daysCholecystokininoctapeptideinducedpancreaticenlargementbyincreasingcontentsofprotein，DNA，RN

10、A，putrescine，spermidineandspermine，alsoactivitiesofamylase，trypsin，andchymotrypsininrats妒005)Correspondingtothechangeofpancreas，serumNO，cGMPcontentsandconstitutivenitricoxidesynthase(cNOS)activitiesshowedasimilarpatternt

11、hatcholecystokininoctapeptideincreasedcontentsofNOandcGMPandtheactivitiesofcNOSofseruminratsreceivingcontinuousadministrationofcholecystokininoctapeptideHoweveralleffectsofcholecystokininwereabolishedbynitricoxidesynthas

12、einhibitorInsummarycholecystokininoctapeptideinducedpolyaminedependentpancreatichypertrophyandhyperplasia，andalsoelevateddigestiveenzymeactivitieswhichrelatedwithNO／cGMPsignalpathwaysInexperiment5，pancreaticacinarcellsob

13、tainedfromthreeratswereincubatedwitheithersaline，orcholecystokininoctapeptide(100fmol／mL)andsoybeanagglutinin(1000fmol／mL)aloneorincombinationAmylase，trypsin，andchymotrypsinactivitiesfrombothculturemediaandacinicellswere

14、stimulatedbysoybeanagglutininandthecholecystokininoctapeptidealoneorincombination(尸005)，andenzymeactivitiesincholecystokininoctapeptidetreatmentwerehigher，butlowerinsoybeanagglutinintreatmentthanthoseincombinationInconcl

15、usion，soybeanagglutinininducedpancreaticenzymerelease，mainlythroughcholecystokinin—mediatedratherthandirectpathwayandsoybeanagglutinininhibitedcholecystokininintermsofpancreaticenzyrmereleaseKeywords：Soybeanagglutinin，Ch