大鼠全腦缺血海馬區(qū)Nestin和bFGF表達(dá)的研究.pdf

1、Objective: To observe mobile changes of Nestin and basic fibroblast growth factor (bFGF) expression in hippocampal after cerebral ischemia and investigate possible mechanism of intrinsic neurogenesis.Method A total

2、 of 40 Sprague-Dewley(SD) rats were divided into control group(4),experimental groups(36) randomly. Experimental groups were divided into 3、 6、 12、24hours and 3 、 7、 14、 21、 28 days after reperfusion. The model of experi

3、mental ischemia was made by 4-VO. The specimens were made into paraffin section. The expression of Nestin and bFGF in hippocampal zone were detected by immunohistochemistry method.Results (1) Nestin detectation: No Nest

4、in positive cells were observed in hippocampal zone in control group. The expression of Nestin started at 3h in SVZ not in hippocampal zone and it started to increase at 6h after cerebral ischemia and reach a peak at 14d

5、 after cerebral ischemia in hippocampal zone, then decreased at 21d,28d. The difference of Nestin expression among the adjacent givengroups is significant, (p＜0.05).(2)bFGF detectation: A few bFGF positive cells were obs

6、erved in hippocampal zone in control group. The expression of Nestin started to increase at 3h in hippocampal zone after cerebral ischemia and reach a peak at 7d after cerebral ischemia in hippocampal zone, then decreas

7、ed at 14d,21d. At 28d it decreased to normal level.The difference of bFGF expression among the adjacent givengroups is significant, (p＜0.05).Conclusion(1) The expression of bFGF and Nestion in hippocampal zone after cere

8、bral ischemia increased, which may be a beneficial response of cells to the ischemic injury. Protective funct ion(2) The sequence of expression of bFGF and Nestion in hippocampal zone after cerebral ischemia is relevant.