非病毒基因載體介導(dǎo)的siRNA.pdf

1、遼寧師范大學(xué)碩士學(xué)位論文非病毒基因載體介導(dǎo)的siRNA姓名：金文實申請學(xué)位級別：碩士專業(yè)：動物學(xué)指導(dǎo)教師：林君201204ResearchofsiRNAmediatedbyNonvirusvector、Abstract—RNMismediatedbythenon—virusvectordsRNAismediatedintothecancercells，resultinbreakingdownthemRNA，whichmakereleva

2、ntgenenotexpress，SOtheprocessisalsocalledgenesilencingItisoneofthemostbriliantmethodsingene—therapyofgenaticlevelsCurrentlythis：methodisgenerallyusedinmicologyzoology。an—d，botallyTheadVantageofRNAiisthathasbettercharacte

3、ristic，usesimplelyand，囊conyinientlytransferefficientlySOandonNowthatitiswidelyusedaSestimatingthe0functionofgene，genetherapyandmedicalselection，whichexertimportantj≥effectThroughtheuseofsmallinterferingRNAwhosesizeissepe

4、ratedtO21_25疊0nucleotidesfromRNAipereventssomespecialgeneexpressingThisdessertation。renewedthegenethreapygenevectorliposome，theinnerprocessofthecomponentofjcationiclip0SIsomeandDNA，RNAi，siRNA，plasmidAn小selectthecationicl

5、iposom，e。0C’／D趾dLipofectamine2000asgenevector；throughusingtheluciferasegenedotheexperimentofRNAiNextf0110wsthemainprocessofexperimentandconsequence|』『I|『_C／DandLipofectamine2000transferredtheplasmidDNAintotheHelajcellsob

6、servingtheefficiencyoftransfectionSecondlyresearchedmoreaboutmedelayingreactionofthecomponentbetweencatiomcliposomeandsiRNA；inj：additionDifferentconcentrationssiRNAwereusedtosilence1uciferasegene’s璺ct，ivitytheresultwasex

7、aminedbymicroplatereaderAndMTTassaywasusedtoanalyzethecellsviabilitywhichwere觚sfected||AsaresultC／Dan畦Lipofectamine2000couldefficientlytransfertheplasmidiyDNAandhadabindingabilitywithsiRNAThelowerconcentrationofosiIu呵A，e

8、xpressed。thehighefficentinsilencinglevelandaffectedlittletocell，一j—viabilityThe：survivalrateofcellsweredecreasingwhiletheconcemtrationofsiRNAwasinceasingInconclusion，catiomcliposomeC／Dand。Lipofectamine2000Cantransferalit

9、tle10werconcentrationsiRNAtosilencetargetgene’SactivityjefficientlyCationicliposomeC／Dcansilenceluciferasegenemoreefficientlybut，、causeahigercellCytotoxicityByoptimizingtheexperimentalconditions，cationicliPosomecalltrans