醋酸棉酚對小鼠精母細(xì)胞中染色體配對行為的影響.pdf

1、河南大學(xué)碩士學(xué)位論文醋酸棉酚對小鼠精母細(xì)胞中染色體配對行為的影響姓名：代小華申請學(xué)位級別：碩士專業(yè)：遺傳學(xué)指導(dǎo)教師：劉靜宇20050601AbstractSynaptonemaicomplex(so)isatransientstructurewhichhappenedineukaryoticnucleiduringthefirstmeioticdivisionwhenhomologouschromosomesarepairingThes

2、tructureiscloselyrelatedwithhomologouschromosomessynapsis，recombinationandsegregationThestudiesonSCmaynotonlycontributetotheknowledgeofsubmicroscopicstructureinmeiosisbutalsohasbeenwidelyusedinthestudiesoneukaryoticchrom

3、osomerearrangement，medicalcytogeneticsandgenetictoxicologyGossypolwasreposedmaleantifertilityagentbyChinesescientistsatfirstIthasnotbeensystematicallystudiedifthegossypolresultedintheaberrationofSCTheconventionalmethodsw

4、ereapplmdinpreparingchromosomesfrommarrowcellandsexcell，TheSCswerepreparedbysurfacespreadingsilversstainingtransmissionelectronmicroscope(TEM)techniqueThestructureandpairingofchromosomesandSCsfromcontrolmalemice(untreate

5、dbygossyp01)andexperimentmice(treatedbygossyp01)wereobservedbylightmicroscope(LM)andTEMTheresultsarereposedasfollows：(1)TheSCkaryotypew0sconstructedinspermatocytesofuntreatedmiceagreedcloselywiththatofmimticchromosomes(r

6、elatedcoefficientofmeanrelaivelengthswas09754)，ItshowedthatSCskaryotypeanalysiswasimportanttecognitionchromosomalaberrationsinspermatogenesisItwasoneofthemostimportantmethodsinstudymaleinfertility(2)Mitoticmetaphasechrom

7、osomesinuntreatedmousewereidentifiedhyanewchromosomalfluorescencebandingtechniquecombining4’，6diamidino2phenylindole(DAPI)stainingwithimageanalysisClearDAPImultiplebandslikeGbandscouldbeproducedBasedontheresults，theclear