應(yīng)用PCR-DGGE技術(shù)研究大麻漚麻系統(tǒng)中的細菌多樣性.pdf

1、黑龍江大學(xué)碩士學(xué)位論文應(yīng)用PCRDGGE技術(shù)研究大麻漚麻系統(tǒng)中的細菌多樣性姓名：江云飛申請學(xué)位級別：碩士專業(yè)：微生物學(xué)指導(dǎo)教師：蔡柏巖20090401黑龍江大學(xué)碩士學(xué)位論文ii一ii暑iiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiii苗i宣iiiiiiiiiii—‘’AbstractInordertofindthedominantflorainthehemprettillg，andlaythefundamentfo

2、rbreedinghempgummingstrains訪thbacteriarettingtechnology，thebacteriuminhemprettingwaterwereusedtoanalysisforbiodiversity24bacteriastrainswereisolatedfromhemprettingwaterof10differenttimesbytraditionalcultivationThestrains

3、wereidentifiedasmembersof11generasand15speciesaccordingtothemorphological，physiological，biochemicalcharacteristicsandthesequencingof16SrDNA：Bacillusmegaterium，Bacilluscereus，Bacilluslicheniformis，Bacillusanthracis，Shigel

4、lasonneiShigelladysenteriae，Pseudomonasargentinensis，Exiguobacteriumsp，EscherichiacotiPaenibacillussp，Klebsiellapneumoniaesubsp，Kurthiagibsonii，Enterobacteriaceae，Lactococcuslactissubsp，PantoeaagglomeransTheV3domainseque

5、ncesof16SrDNAfrombacteriumin10differenttimesinfermentetionliqmdofhemprettingwereamplifiedbyPCRTheoptimumconditionofDGGEwasfinallyconfirmedthatelectrophoreseswererunfor8hat150Vona8O％aerylamide／bis—acrylamide(375：1)gel、Ⅳit

6、h40％一70％denaturantgradientAllDGGEanalysiswasrunataconstanttemperatureof60“0DGGEsequencinganalysisshowthatthedominantbandswere：Chryseobacteriumsp，Listeriagraft，Pantoeaagglomerans，Serratiamarcescens，Klebsiellapneumoniae，En

7、terobactersp，Stenotrophomonasmaltophilia，Pediococcuspentosaceus，Clostridiumsp，LactococcuslactissubspandunculturedbacteriumDGGEfingerprintwasanalyzedbyGelProAnalyzer45Diversityindexrevealedthatthedominantfloraweremoreobvi