發(fā)酵法降低棉粕中游離棉酚含量及提高其營養(yǎng)價(jià)值的研究.pdf

1、華中農(nóng)業(yè)大學(xué)碩士學(xué)位論文發(fā)酵法降低棉粕中游離棉酚含量及提高其營養(yǎng)價(jià)值的研究姓名：聶蓬勃申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：發(fā)酵工程指導(dǎo)教師：梁運(yùn)祥湯江武20080601發(fā)酵法降低棉粕中游離棉酚含量及提高其營養(yǎng)價(jià)值的研究AbstractCottonseedmealisoneofhighqualityplantproteinresources，andthecrudeproteincontentofitisabout40％Butthefreegossyp

2、olandotheranti—nutritionalfactorsincottonseedmealhavebadlyinfluenceditsfeedvalueThissubjectemployedmicroorganismfermentationmethodteducefreegossypolandothertoxicsubstances，degradetheproteinincottonseedmealtopeptidesandpr

3、oteinswithsmallmolecularweightwhichcouldbeabsorbedeasilybyanimals，accumulatedsomebenefitproducts，andthenimprovedthenutritionalqualityofthecottonseedmeal，aswellasincreasedthedosageaddedtothefeedstuffBasedonthefoundationof

4、analysismethods，somestrainsusedinthisfermentationwereobtainedfromtheorganismsreservedbythelaboratoryAstrainnamedB2couldsignificantlyreducethecontentoffreegossypol；itWasidentifiedasBacilluscereusAnotherstrainnamedB1coulds

5、ignificantlydegradethecottonseedmealproteintosmallpeptidesidentifiedasBacillussubtilis；twostrainsnamedY2andLwhichcouldbringgoodsmellanddaintywerealsoscreened(StrainY2isayeast；strainLisalactobacillus)Solefermentationresea

6、rchonstrainB2Wasadoptedtoseetheeffectondetoxificationratiooffreegossyp01Thebestprocessparameterswereconfirmed：FermentationdurationWas48h，feedtowaterratioWas1to1，inoculumsdosewas2％，temperatureWas28℃andinitialpHWasjustinna

7、turestateOnthisconditionthedetoxificationratiocouldreachtO519％Also，solefermentationresearchonstrainB1WasadoptedtoseetheeffectondecompositionofcottonseedmealproteinThebestprocessparameterswereconfirmed：Fermentationduratio

8、nWas72h，feedtowaterratioWas1to1，inoculumsdoseWas4％，temperaturewas30。CandinitialpHWasjustinnaturestateOnsuchcondition，theTCANSIcouldreachto330％Basedonthestrainsscreenedfromtheorganismsreservedbythelaboratoryandthesoleferm

9、entationresearch，mixedfermentationresearchWasadoptedFinallytheoptimumcombinationforfermentationWasB1B2Y2LInoculumsdoseofeachorganismis1％(grossweight)，fermentationdurationWas48h，feedtOwaterratioWas1to12，temperatureWas30“C