化膿菌腸道菌分離鑒定過(guò)程四

1、化膿菌/腸道菌分離鑒定過(guò)程（四）,,甘露醇發(fā)酵試驗(yàn)藥敏實(shí)驗(yàn),,細(xì)菌的抑菌環(huán)直徑解釋標(biāo)準(zhǔn)和相應(yīng)的MIC解釋標(biāo)準(zhǔn)（來(lái)源于CLSI M100-S17和NCCLS M31-A2）,1：腸桿菌科 2：葡萄球菌屬 （2-1凝固酶陽(yáng)性葡萄球菌，2-2凝固酶陰性葡萄球菌）3：腸球菌屬,Bacteriological Detection,,Serological diagnosis,Samples: Widal’s test ---

2、--- S. typhi and paratyphi Weil-Felix Test ------ Richettsia(立克次體屬),Widal’s Reaction,Definition: a specific reaction consisting in agglutination of typhoid bacilli or paratyphoid bacilli when mixed with ser

3、um from a patient having typhoid fever or other salmonella infection and constituting a test for the disease .Usage: to assist to diagnosis typhoid fever or other salmonella infection.,Materials,Antigens:

4、 TO TH PA PBAntibody: serum of suspected patientNormal salineReaction plateStraws,PROCEDURE,Make the markAdd reagents as the following: Reagent (ml) 1 2 3 4 5 6 7

5、,,,,,Final serum 1:40 1:80 1:160 1:320 1:640 1:1280 - dilution,Shaking several times, put it in 37℃ water bath for 2 hours.Then let it stand at room temperature over night.,Bacteria suspensio

6、n: TO TH PA PB,Observation:,1. Control tube: no agglutination (-) 2. Lowest titer tube: absolutely agglutination (++++) 3. Other tubes: three quarters agglutination(+++) half agglutination

7、 (++) one quarter agglutination (+) no agglutination (-)Judgment: Agglutination titer: the highest dilution of serum which appears (++) bacteria agglutination.,H抗體與O抗體的性質(zhì)及其消長(zhǎng)的意義,O抗體,I

8、gM,出現(xiàn)較早維持時(shí)間短,H抗體,IgG,出現(xiàn)較晚維持時(shí)間長(zhǎng),傷寒的可能性很大,,高,高,高,低,感染早期,O凝集的交叉反應(yīng),低,高,預(yù)防接種 非特異性回憶反應(yīng),,,,,,抗體類(lèi)型,體內(nèi)出現(xiàn)情況,與正常值比較,Analysis,Precautions,Make the markMake sure the procedurecarefully use the strawsCorrectly Judg

9、e the results,細(xì)菌特殊結(jié)構(gòu)觀察,莢膜莢膜染色法：菌體紫色，莢膜無(wú)色或淡紫色芽孢芽孢染色法：菌體藍(lán)色，芽孢紅色鞭毛鞭毛染色法：菌體紅色，鞭毛紅色異染顆粒異染顆粒染色法：菌體淺黃色，異染顆粒深藍(lán)紫色抗酸染色抗酸性細(xì)菌：紅色非抗酸性細(xì)菌：藍(lán)色,模擬題,在SS瓊脂平板上，致病性腸道桿菌菌落特征是____________，非致病性腸道桿菌的菌落特征是____________。斜面培養(yǎng)基主要用于 _______