爽咽茶抗菌試驗(yàn)研究

1、爽咽茶抗菌試驗(yàn)研究爽咽茶抗菌試驗(yàn)研究爽咽茶抗菌試驗(yàn)研究更新日期：20100608點(diǎn)擊：龍子江馬建元呂曉英李守好摘要：采取試管法進(jìn)行體外抗菌試驗(yàn)，證明爽咽茶對(duì)金黃色葡萄球菌、肺炎球菌、甲鏈、乙鏈大腸肝菌及肺炎克雷伯菌等有明顯的抑制作用；對(duì)金黃色葡萄球菌的作用最強(qiáng)，MIC50為3.03125gml；對(duì)大腸肝菌作用最差，MIC50為1gml。體內(nèi)抗菌試驗(yàn)測(cè)得爽咽茶對(duì)金黃色葡萄球菌和肺炎克雷伯菌感染小鼠有明顯的保護(hù)作用，其ED50分別為3.36

2、5gkg和4.189gkg。關(guān)鍵詞：@爽咽茶藥理學(xué)；金黃色葡萄球菌藥物作用；肺炎球菌藥物作用；金黃色葡萄球菌微生物學(xué)；肺炎球菌微生物學(xué)中圖分類號(hào)：R285.5；R378.1文獻(xiàn)標(biāo)識(shí)碼：A文章編號(hào)：10039783(1999)06036002AStudyonAntimicrobialEffectofShuangyanTeaLONGZijiangMAJianyuanLUXiaoyingetal(DepartmentofPharmacolog

3、yAnhuiCollageofTCMHefei230038)Abstract:AntimicrobialtestinvitroshowedthatShuangyanTeacanconsiderablyinhibitthegrowthofstaphylococcusaureusDiplococcuspneumoniaeαStreptococcusβStrepEscherichiacoliKlebsiellapneumoniae.Thebo

4、cteriostatiseffectanStaphylococcusaureusismostpotentMIC50is0.0325gmlwhilethatonEscherichiacoliislowestMIC50is1gml.AntinricrolialtestinvivoshowedthattheteacanevidentlyprotectthemiceinfectedbyaureusklebsiellapneumoniaeED50

5、being3.002gkg4.189gkgrespectively.Keywds:@ShuangyanTeapharmacologyStaphylococcusaureusdrugeffectpneumococcusdrugeffectStaphylococcal0.063甲鏈50.2500.3750.250～0.5000.2500.3750.250～0.500乙鏈50.1250.2500.125～0.2500.2500.2500.12

6、5～0.250肺炎球菌50.5000.5000.500～1.0000.2500.5000.250～0.500大腸桿菌51.0001.0000.500～1.0001.0001.5000.125～2.000肺炎克雷伯菌50.1250.1880.125～0.2500.2500.2500.125～0.2502.2體內(nèi)抗菌試驗(yàn)［1］2.2.1對(duì)金黃色葡萄球菌感染小鼠的保護(hù)作用體重18～22g小鼠60只，雌雄兼用，隨機(jī)均分成6組；每鼠ip0.5ml

7、菌液(細(xì)菌濃度為4107CFUml)，感染小鼠，于感染后按表2中劑量ig給藥，正常飼養(yǎng)，連續(xù)觀察7d，記錄小鼠死亡數(shù)，根據(jù)試驗(yàn)結(jié)果，用Blisss法計(jì)算出對(duì)感染小鼠保護(hù)作用的半數(shù)有效量(ED50)和95％的可信限，結(jié)果見(jiàn)表2。表2爽咽茶對(duì)金黃色葡萄球菌感染小鼠的保護(hù)作用組別劑量(g.kg1)N只存活數(shù)只存活％ED5015.000109903.36524.0001077033.200104402.96642.56010330～52.048