45333.唐魚卵黃蛋白原cdna克隆及其表達(dá)調(diào)節(jié)的研究

1、華南師范大學(xué)碩士學(xué)位論文唐魚卵黃蛋白原cDNA克隆及其表達(dá)調(diào)節(jié)的研究姓名：王瑞龍申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：動(dòng)物學(xué)指導(dǎo)教師：馬廣智20060501華南師范大學(xué)碩士學(xué)位論文AbstractEnvironmentalestrogenshavegreatlythreatentheexistingofwildlifeandhealthofhumanbeingsMethodsforscreeningenvironmentalestrogenhaVeb

2、eenstudiedandso吼eofthemhadbeenusedbroadlyVitellogenin(1門G)hasbeenstudiedextensivelyasabiomarkerforthescreeningofenvironmentalestrogen，becauseofitshighspeciality，highsensitiVityandconVenienceforsampling71a，7j曲盤prsa』6D門“6P

3、sisasmallcyprinidfishofDanioninaeFewstudieshavebeendoneexceptstudiesaboutartificialreproductionandtoxicityatpresentNostudieshavebeenconductedonusingVTGofZaJ6D門曲P占todetectenvironmentalestrogenTheVTGcDNAwasis01atedFromthel

4、iveroffemaleZaJ619刀u6呂sgrouperbyPCR，5’RACEand3RACEtechn0109yTheVTGcDNA，4171bpinsize，encodesapolypeptideof1326aminoacidThesequenceanalysisshowedthatthePi月7Pp方四』esp，D口糟』asVTGsharedhighhomologywith￡曰rf，7Usca印jDa門d如，7，。，P，，o

5、VTGs，87％，85％and84％respectivelyataminoacid1evelThetissuedistributionofVTGmRNAwasexaminedusingRT—PCRTheresultsshowedthehighestexpressioninthe1iverItisalsoexpressedinextrahepatictissue，suchastheovary，thebrain，gillandtestis，

6、butnoexDressioninthemuscleTheinductionofVTGmRNAinmaleZa』60門“6Psbydippingwith17一Bestradiolforfourteendayswasanalysedwithsemi—quantitativeRT—PCRTheresultsshowthattheexpressionofvTGⅢRNAinmaleZa』6D門如Pswassignificatilyincreas