水稻脂肪氧化酶同工酶缺失基因的RAPD標(biāo)記.pdf

1、寧夏大學(xué)碩士學(xué)位論文水稻脂肪氧化酶同工酶缺失基因的RAPD標(biāo)記姓名：高山申請學(xué)位級別：碩士專業(yè)：作物遺傳育種指導(dǎo)教師：陳素生吳躍進(jìn)20050301Abs打actResearchesinrecentyearsindicatedthatthelipoxygenase(LOX)(ECl131l，12)isthekeycnzymeofthericelipiddeteriorationTheabsenceofLOXmaythus，atleasti

2、npartreducedegradation，andalleviateseedsaging，andstrengthenthestoragestabilityofriceTherewerethreekindsofLOXs—LOXl，LOX2andLOX3inricechaffandembryoTheabsenceofLOXsisinheritedwimasinglerecessivetraitandtheabsenceofLOXsdoes

3、n’taffectricegrowthTherandomamplifiedpolymorphicDNA(RAPD)analysiswascarriedOUtinwanjiart2090，DawDamanditsnearisogeniclines(NiLs)Themainresultswereasfollows：1ComparedwiththeNILs，theabsenceofLOXdoesn’taffectricegrowth2]hei

4、nfluencingfactorsonRAPDwerestudiedandexperimentalparameterswereoptimizedTheywereaSfollows：20I_tLsystemcontaining50ngtemplate，O20pmol／LprimerlpL25mmol／LdNTP1UnitsTaqDNApolymerase，20mmol／LMgCl2and139LddH20’rheoptimalamplif

5、icationprogramWaSaSfollows：2minat94(2，followedby94“Cfor30sec，365℃30sec，72℃for90see，for40cyclesandfinaltimeof72℃for5min，atlaStsustainat4“CAhi曲reproducibleresultswereobtainedwiththeoptimizedexperimentconditions3ARAPDanalys

6、iswasmadebetweenwanjian2090，DawDamanditsNILswith650arbitraryprimersAfterexperimentedbyseveralRAPDrepetition，sixprimersamplifiexltheDNApolymorphicbandsintheNILs，linkedcloselywiththericeLOXs—nullgenesThehereditybackgroundl

7、ikenesswereveryconsistentbetweentheNILsTwopolymorphicRAPDmarkerslinkedticeembryoLOXlnullgenewerescreened，S1302797andS1518530；RAPDmarkerlinkedticeembryoLOX2nullgene，S1509683；RAPDmarkerslinkedtodeeembryoLOX3nullgene，S12269