纖維質(zhì)酒精發(fā)酵的菌種選育及發(fā)酵條件的研究

1、吉林農(nóng)業(yè)大學(xué)碩士學(xué)位論文纖維質(zhì)酒精發(fā)酵的菌種選育及發(fā)酵條件的研究姓名：任立偉申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：生物物理學(xué)指導(dǎo)教師：陳光20030601吉林農(nóng)業(yè)大學(xué)碩士學(xué)位論文纖維履酒精發(fā)酵的菌種選育及發(fā)酵條件的斫究AbstractThispaperstudiedthetechnologicalprocessoffermentingcellulosetOfuelethanolbysaccharomycescercvisiaeYT318whichwa

2、sobtainedthroughUVmutationComparingtOoriginalsaccharomyceteYTl8，YT318increasedinethanolproductioncapacityby15％anddecreasedinfermentationwith12hTheoptimizedconditionsofpreparingcellulosewithvapour—explodingwas：pressureat1

3、2Mpa，time5min，productivity6772％SystematicresearchonlaterpartofalcoholfermentationandnutrientfactorswasconductedItfoundthatYT318hadgreatcapabilityofalcohol—productiononglucoseandxyloseExcessiveglucosedensity，toxiousmateri

4、al，temperature，acidandSOoncouldinhibitfermentationSomefactorstookeffectinearlierpartoffermentationwhileothersinlatterpartButthesynergeticeffectsofthesefactorsinhibitedfermentationevenmoreEarlierpartoffermentationwereinhi

5、bitedat10％totalsugarconcentrationLowsubstratedensitywasbeneficialtOimprovementofethanolOUtpUtUtilizationratio0nrawmaterialcouldincreasefrom8262％to8851％infed—batchfermentationSoitshowedsubstrateinhibitioninearlierpartoffe

6、rmentationtookmoleeffectthatalcoholtoxicityinlatterpartoffermentationAddingofnitrogensourcehelpedproducealcoholandwouldshortenfermentationperiodAreawasthebestinorganicnitrogensourcewhichshouldmaintainathi曲concentrationat

7、earlierpartoffermentationbecauseofsaccharomycete’SfeatureOxygenwasbeneficialtOcellgrowthandlivingfactorsproductionItwouldenhancethefermentationiffixedamountofoxygenwasmaintainedAsthebestsaccharomycetetopreparedcornstalk，