油菜籽皮發(fā)酵纖維素酶菌種的選育及條件優(yōu)化.pdf

1、湖北工業(yè)大學(xué)碩士學(xué)位論文油菜籽皮發(fā)酵纖維素酶菌種的選育及條件優(yōu)化姓名：胡婷婷申請(qǐng)學(xué)位級(jí)別：碩士專(zhuān)業(yè)：生物化工指導(dǎo)教師：邱雁臨20080501湖北工業(yè)大學(xué)碩士學(xué)位論文AbstractCelluloseisthelargestrenewableresourceontheearthThecontentofcelluloseinrapeseedhullachieves31％一34％andastheoutgrowthintheprocessofm

2、achiningedibleoilrapeseedhullisasortoflatentrenewableresourcewhichyoucanbenefitfromeconomicallyTherefore，tocontriveanewapproachtotransformingandutilizingrapeseedhullincreasinglyhasawidestforegroundThestudyachievesasortof

3、additivewhichcontainshigh—techandadditionalgainsandfermentswithrapeseedhullprincipallyAndthisopensupaneffectiveareaofutilizingrapeseedhullsyntheticallyBythesinglefactortest，thepaperattainedtheoptimalcompounding：cultureag

4、e：20h，degradationtemperature：32℃anddegradationtime：3hOnthisconditiontherateofregenerationtoprotoplastformation，was10％一15％；webredamutantstrainUVTl2whichobtainedbymutagenizingtheprotoplastoforiginalstrainTowithultravioletr

5、adiationandtheCMCactivityofcellulasereach204049u／gfreshsubstrate，increased1243％toTo；bredamutantstrainlswhichobtainedbymutagenizingtheprotoplastofori百nalstrainUⅥ12withLiCIandtheCMCactivityofcellulasereach235848u／gfreshsub

6、strate，increased146％tostrainUⅥ12；bredamutantstrainS6whichobtainedbymutagenizingtheprotoplastoforiginalstrainUⅥ12withDESandtheCMCactivityofcellulasereached325064u／gfreshsubstrate，increased3713％tostrainUVTl2and2608％tostrai

7、n，11nBythesinglefactortest，Lls(3’)orthogonaltestofsubstrateandL9(34)orthogonaltestoftheconditionoffermentation，weobtainedtheoptimumcompoundingofthefactolsoffermentationtomutantstrainS6：rapeseedhull：straw：branis5：2：3，thed

8、rysubstrate：water1：1，(NH4)2S041％，KH2P0404％，MgS040，05％，CaCl2006％，tween80015％，thevolume10％，theinoculumconcertration4％，thetemperature32℃andthetime72hUndertheseconditions，theCMCactivityofcellulasetomutantstrainS6reached43636

9、4u／gfreshsubstrateandincreased342％thanbeforeWeworkedoverthefermentationwithmutantstrainS6andAspergillusniger856mixedBythesinglefactortestandI9(3’)orthogonaltestweobtainedtheoptimumcompoundingofthefactorsoffermentation：th

10、etemperatureis30℃，Aspergillusniger：Trichodermaviride1：1。thevolume10％theinoculumconcertration4％UnderthCSCconditions，theCMCactivityofcellulasereached481413u／gfreshsubstrateandincreased103％thanthefermentationwithmutantstrai