雜合抗生素生技霉素和桔青霉68活性代謝產(chǎn)物的化學(xué)研究.pdf

1、中國協(xié)和醫(yī)科大學(xué)中國醫(yī)學(xué)科學(xué)院博士學(xué)位論文雜合抗生素生技霉素和桔青霉68活性代謝產(chǎn)物的化學(xué)研究姓名：姜威申請學(xué)位級別：博士專業(yè)：微生物與生化藥學(xué)指導(dǎo)教師：金文藻19990601PART1ISOLATlONANDSTRUCTURElDITIFICAITONOFMINORCOMPONENTSOFSHENGJIMYClNSABSTRACTShengjimycinsareagroupofhybrid4“Oacylspiramycinswhich

2、wereproducedbyageneticallyengineeredstrainWSJ1bearing4“OisovaleryltransferasegeneShengjimycinshaveshoweds打ongactivityagainstgram—positivebacteriaandsomegramnegativebacteriaItismoreactivethanclinicallyused4“0一ace印lspiramy

3、cinanderythromycininvivoShengji—mycinsarehopefulbioengineeredantibioticsforclinicalusageThefirstpartofthisdissertationismainlyonthechemicalstudyofminorcomponentsofShengiimycinsHPLCanalysisdemonstratedthatShengjimyeinswer

4、emulticomponentantibioticsThemajorcomponents，ShengjimyeinAl，BlandE1whichprovedtobe4”一O—isovalerylspimmycinⅢ，IIandIpreviously，hasaccountedforabout55％ofthemixtureSomeother4“O—acylspiramycinssuchas4“Oacetylspiramycin，4“O—pr

5、opionylspiramycin，4“ObutyrylspiramycinandfewspirarnycinshavealsobeenfoundTotalcontentof4“O—acylspiramycinshasreachedtoabout90％ofthecrudeproductBymeansofcontinuouscountercurrentdistribution，basicsilicagelcolumnchromatogra

6、phy，preparativeTLCandmediumpressureliquidchromatographymonitoredbyTLCandHPLC，fiveminorcomponents，ShengjimycinC2，B3，B2，Boand氏wereisolatedfromShengjimycinscrudepowderthecontentsofthemwere26％，62％，33％，15％andO5％，respectivelyS

7、henNimycinB2isacomplexofB2。andB2口isomerBasedoncarefulanalysisofUv，IR，SIMS，F(xiàn)ABMS，GCMS，1HNMR，”CNMR，1HJHCOSY，thestructuresofShenNimycinC2，B3，B2。，B2口，BoandAowereelucidatedas4“一O—propionylspiramyeinII4“0一propionylspiramycinII