柑桔內(nèi)生細菌對油菜菌核病菌的拮抗作用研究.pdf

1、湖南農(nóng)業(yè)大學碩士學位論文柑桔內(nèi)生細菌對油菜菌核病菌的拮抗作用研究姓名：冉國華申請學位級別：碩士專業(yè)：植物病理學指導教師：張志元20050601StudyontheantagonismofcitrusendophyticbacteriaAbstractAdoptingthemethodoforganizeseparating，86bacterialisolateswereobtainedfromGuangdong，Chongqing，Hu

2、nancitrusretlulataBlanco，citrussinensisOsbeckandCitrusgrundisOsbeckeveryorgan(cortexofmainstem，cortexofmainbranch，leaf，fruit，fibrousroot)，hadunderstoodthedistributionsituationoftheendophyticbacteriaofculturemandarinorang

3、eofourcountrypreliminary：accordingtotheregions，thebacteriabearingrateinGuangdong，Chongqing，Hunanthreeplacehealthorganizationofmandarinorangesrespectivelyis21296。16O％and153％，thetendencywasteducegraduallyfromthesouthtothen

4、orth：Accordingtokinds，thebacteriabearingrateofcitrussinensisOsbeckwasthehighest，itwas237％secondlyitwas188％ofcltrusretiulataBlancoC1trusgrundisOsbeckwas10O％：accordingtoeveryorgan，thehighestbacteriabearingratewasthefibrous

5、root。was417％，secondlyitwasleaf303％，cortexofmainbranchwas84％，cortexofmainstemwas61％，endophyticbacteriainfruitonlywas11‰Throughtheplateformantagonismtest，35bacteriawerescreenedwhichhadantagonismtoSclerotinlasclerotiorum6am

6、ongtheminhibitingratewereabore50％：2bacteriastillhadstrongerantagonismaftertheirbacteriasuspendliquiddeactivatedin110℃for20minutesChoosed03103221and03112121twobacterialstrains，whichbacteriasuspend1iquidandbacteriadeactiva

7、tedliquidhadbetterantagonismtoSclerotiniasclerotlorum，25～30℃shakingtrain72husingpotatocanesugarcultureliquidmadtofermented1iquid，carriedonrapedetachedleafinhibitingtestandfieldpreventdiseaseexperiment，resultsshowedthat03