實(shí)時(shí)熒光定量PCR檢測(cè)肺癌患者血漿循環(huán)DNA及其臨床意義.pdf

1、Y35a007分類號(hào)：[R34]學(xué)校代碼：10392福建醫(yī)科大學(xué)碩士研究生畢業(yè)論文實(shí)時(shí)熒光定量PCR檢測(cè)肺癌患者血漿循環(huán)DNA及其臨床意義ReaitimeFluorescenceQuantitativePCRinDetectionofCirculatingDNAinPlasmafromPatientswithLungCancerandItsPotentialClinicalSignificance所在院(系)：基礎(chǔ)醫(yī)學(xué)院研究生：溫開鎮(zhèn)學(xué)

2、科(專業(yè))：生化與分子生物學(xué)導(dǎo)師：林德馨教授張忠英教授研究起止日期：2005年3月至2006年4月二00六年四月RealtimeFluorescenceQuantitativePCRinDetectionofCirculatingDNAinPlasmafromPatientswithLungCancerandItsPotentialClinicalSignificanceAbstractPurposeToquantifythecircu

3、latingDNAinplasmafrompatientswitlllungcancerandevaluateitspotentialclinicalsignificanceMethodsBloodsampleswerecollectedfrom80patientswithlungcancer40benignlungdiseasepatientsand97healthycontrolsPlasmaDNAwasextractedandpu

4、rifiedby“genomicDNAextractionkit’ThequantitationoftheplasmaDNAwasperformedbyrealtimefluorescencequantitativePCRassayScreeningperformanceoftheassaywascalculatedthroughthereceiveroperatingcharacteristic(ROC)curveResultsThe

5、medianconcentrationofcirculatingplasmaDNAinlungcancerwas1985ng／ml，whichwassignificantlyhighercP=O000)thanthatofthepatientswithbenignlungdisease(46ng／m1)andthehealthycontrols(32ng／m1)EarlyinstageI，theamountofcirculatingpl

6、asmaDNAhasincreasedto147ng／ml，whichwasthreetimeshigherthanthepatientswitlbenignlungdiseaseandfourtimeshigherthanhealthindividualsfP=O003)TheareaundertheROCcurvewasO879(95％CI，0830～0928)Withathresholdvalueof1075ng／ml，theov

7、erallsensitivityoftheassaywas826％specificitywas847％ThemedianconcentrationofcirculatingplasmaDNAwascloselyrelatedtoitscTNMstage，tumorsizeandserumCYFRA21—1(P=0028，P=0027，P=0006)，butnottothepathologicaltype，t121TIortype，his

8、tologicalgradeandleukocytecounts(P=O786，P=0658P20772P20068)ThemedianplasmaDNAconcentrationinpost—surgerysampleswassignificantlylowerthanthatinpre—surgewsamples(P=0001)，butnodecreasingorincreasingwasfoundinpatientswithcan