UC--MSCs歸巢對CIA大鼠免疫炎性易栓癥的調(diào)控作用.pdf

1、課題來源國家自然科學(xué)基金(81270590)項目名稱：UCMSCs靶向修復(fù)非炎性血栓形成的免疫調(diào)控機(jī)制和體內(nèi)轉(zhuǎn)歸項目主持人：顧健教授主任醫(yī)師江蘇省衛(wèi)生廳醫(yī)學(xué)科研立項課題(H201048)項目名稱：磁性納米粒子示蹤MSCs靶向修復(fù)RA關(guān)節(jié)損傷的機(jī)制研究項目主持人：顧健教授主任醫(yī)師揚州大學(xué)碩十學(xué)位論文2TheregulatoryeffectofUCMSCshomingonimmuneinflammatorythrombophiliainco

2、llageninducedarthritisratsAbstractobjecfive：ToresearchtheconnectionofimmuneinflammatorydisorderandimmuneinflammatorythrombophiliainthecollagentypelIinducedarthritis(CIA)rats，andexploretheregulatoryeffectofumbilicalcordme

3、senchymalstemcells(UCMSCs)homingonimmuneinflammatorydisorderandthrombophiliaToresearchtheimpactofinflammatorydisordertotheprothromboticintheCIArats，andUCMSCshomingregulatetheimmuneinflammatorydisordersandthrombophiliasta

4、tusMethods：144(Sprague—DawleyRats)SDratswererandomlydividedintocontrolgroup(groupC)，modelgroup(groupM)，UCMSCstreatinggroupwhichlabledbySPION(groupsu)，thegroupaddedantagonistAMD3100tolabeledUC—MSCs(groupASU)Exceptforgroup

5、C，theotherratswereestablishedmodelofCIAARermodelingiscompleted，thetreatinggroupwereinjectedthesuspensionofUCMSCs，taggedUC—MSCsandtaggedUCMSCsCOculturewithantagonistviathetailveinrespectivelyAftertheratswereinjectedUCMSCs

6、weresacrificedinthefirst，third，fifthweek，andweextracttheabdominalcavityvenousbloodandremovethefreshkneetissueTheconcentralJonofInterleukin10(IL10)Interlenkin6(IL6)，Tumornecrosis缸衄噸㈣F刪，InterferonTOFNT)andtissuefactor(TF)，

7、vonWillebrandfactor(VWF)，Ddimer(DD)，F(xiàn)ibrinogen(FIB)inseraofratsfromeachgroupweredeterminedwiththeenzymelinkedimmunosorbentassay(ELISA)andtheexpressionoflL10，IL6，TNFⅡ，IFN1，inkneescartilageofratsfromeachgroupwasevaluatedby

8、westernblottingResults：1CIAratsjointswellingandulcerationaftersuccessfulmodeling，theiractivitywerelimited，thelevelsofpro—inflammatorycytokinesIL一6，TNF—q，IFN一^ringroupMweresignificantlyhigherthanthecontrolgroupsincethefir