腦外傷后細胞核因子κB（NF-κB）信號通路對自噬過程的調(diào)節(jié)及機制探討.pdf

1、蘇州大學碩士學位論文腦外傷后細胞核因子κB（NFκB）信號通路對自噬過程的調(diào)節(jié)及機制探討姓名：李生興申請學位級別：碩士專業(yè)：法醫(yī)學指導教師：陶陸陽20080501T—heComributionofNuclearFac—tor—KBPath—wayto—AutophagyAncrTraumaticBrain塒uryinRatNF—KBActivatingUp—regualteAutophagicProcessafterTraumaticB

2、rainInjuryinRatAbstractObjection：ToinvestigatethecontributionofNFr。BpathwaytotheautophagicprogressionaftertraumaticbraininjuryinratmodelMethods：TraumaticbraininjurymodelwasinducedbyamodifiedweightdropdevicethatWasallowed

3、afleefalluntilthesiliconetip(diameter5mm)hittheexposedskullontheleftcerebralhemisphere，lmmlateraltothemidlineinthemidcoronalplaneSN50(10Itg)wasiIljectedintracerebroventricularly(icv)lOminbeforetheonsetofstrikeWesternblot

4、WasemployedtodeterminealternationsinBeelinl，Bcl2，cathepsinDandLC3inimpairedcDrtexandhippocampusatdifferenttimepointsafterTBI，aswellasparallelgroupsofmiceResults：TheexpressionofBeclinl，Bcl2，cathepsinDandLC3aresignificantl

5、yincreasedinimpairedcortexandhippocarnpusonlh3hm12h24hand48htimepohnafterTBIAteverypostinjurytime，Beclinl，cathcpsinDandLC3ⅡexpressioninSN50groupislowerwhileBcl2ishigherthanSalinegroupConclusions：1AutophagyWasactivatedbyT

6、BI2NF—vBpathwaycontributestotheregulationofautophagosomeafterTBI3Theexpressionoflysomalenzymecath印sinDWasaccommodatedbyNFr。BpathwayafterTBIKeyWords：Waumaticbrain’injury；autophagy；NFxB；Beclinl；Bcl2；cathepsinD；I，C3ⅡWritten