蔬菜中有機磷農(nóng)藥殘留快速檢測方法的研究.pdf

1、山東理工大學(xué)碩士學(xué)位論文蔬菜中有機磷農(nóng)藥殘留快速檢測方法的研究姓名：王小瑜申請學(xué)位級別：碩士專業(yè)：農(nóng)業(yè)生物環(huán)境與能源工程指導(dǎo)教師：王相友20080420AbstractInordertosolvetheproblemsinrapiddetectiontechnologyfbrpesticideresidues，threepartswerestudiedinthispaper1Comparingthesensitivityofthrees

2、ources(Muscadomestica，cyprinoidsbrain，fowlliver)acetylcholinesterase(AChE)tothreeorganophosphoruspesticides(dichlorvos，trichlorfon，omethoate)ItisfoundthatfordichlorVos，thesensitivityfromhightolowareMuscadomestica，cyprino

3、idsbrain，fowlliVer；fortrichlorfon，thehighestsensitiveAChEisfromMuscadomestica，AChEfromcyprinoidsbrainandfowlliverenzymearenotsensitive；foromethoate，below3OOUgL’1concentration，theinhibitionrateofthreeAChEarelittledifferen

4、ce，whentheconcentrationgreaterthan300pgL～，inhibitionef伯ctofAChEfromMuscadomesticaisbetterthantheresttwoComprehensiveconsiderthesensitivityofthreeAChEtothreepesticides，fromtheMuscadomesticaisbetterininstructionorganophosp

5、hatepesticide2Researchtheimmobilizationef億ctsofAChEfromMuscadomestica0nthethreemicrovoidnlterjFilm(nylon，acetateandnitratefilm)ItisfoundthattheimmobilizedresultsofAChEonnitroceUulosemembraneisthebestAChEimmobilizedonnitr

6、ocellulosemembranewerestudied，thebestimmobilizationconditionsarethat10UofAChE，5uLof5％(volumefraction)glutaraldehyde，30“Lof1％(massfraction)bovineserumalbumin(BAS)，dubbed200肛Lenzymesolution4℃immobilized8h3Theoptimizationof

7、determinationfbrorganophosphatepesticideresidueswasstudiedInpromiseofhighersensitiVity，anewlowcostreagentcomponents，whosedosageofacetylcholinesterase(AChE)，substrateandchromogenicreagentare3／4，3／4and1／2thatoforiginalcomp

8、onentsrespectiVelyF—testproVedthatthereisnosignificantdifferencebetweenadjustedandoriginalcomponentsThelowerdeterminationlimitofad；ustedcomponentstothreepesticidesarethatomethoateO37mgL1，trichlorphon009mgL～，dichlorVos003