正常人及初發(fā)慢性粒細胞白血病白細胞中特定染色體相對端粒長度的研究.pdf

1、華中科技大學碩士學位論文正常人及初發(fā)慢性粒細胞白血病白細胞中特定染色體相對端粒長度的研究姓名：王海霞申請學位級別：碩士專業(yè)：內(nèi)科學(血液病學)指導教師：黃士昂201005華中科技大學碩士學位論文華中科技大學碩士學位論文4MeasurementofChromosomeSpecificRelativeTelomereLengthsinLeukocytesofNmalIndividualsCMLPatientsAbstractObjectiv

2、eToestablisharelativetelomerelength(RTL)basedcommonstardfthemeasurementofsingletelomerelengthsinhumanchromosomesinvestigatethelengthdistributionvariationofchromosomespecifictelomeresofnmalindividualsstudythevariationofch

3、romosomespecifictelomeresofCMLpatients.MethodUsingquantitativefluescenceinsituhybridization(QFISH)thefluescenceintensityofeachsingletelomerewasdetectedthenconvertedtoanRelativeTelomereLength(RTL)thenmakecomparationofthec

4、hromosomespecifictelomeresRTLvaluesamongnmalindividualsbetweennmalindividualsCMLcases.ResultInnmalindividualstelomerelengthsatdifferentchromosomeendshowedanonromdistributionvariedsignificantly(F=8.923，p0.001).TheRTLatchr

5、omosomeendsof2q16p17p17q19p20q22qYqwereshterthanothers（p0.05）whereastheRTLat3p5pXpYp(p:chromosomeshtarmq:chromosomelongarm)werelongerthanothers(p0.05)InCMLcasestheRTLatchromosomeendsof1q3p15p21p22pwereshterthannmalindivi

6、duals（p0.05），whereastheRTLat2q3q6p7q10p10q11p16p17pXpwerelongerthannmalinviduals（p0.05）.ConclusionUsingRTLgeneratedfromfluescenceintensityoftheQFISHtodeterminechromosomespecifictelomerelengthcanbeeasilystardizedanalyzedt

7、he46chromosomes’telomerelengthsshowspecificdistributionprofileinnmalindividualsTherewerespecificlongshttelomeresinCMLpatientsthesetelomeresmaycontributedtotheleukemiagenesisprogressionofCML.［KeyWds］QuantitativeFISH(QFISH