GBEE抑制Lewis肺癌腫瘤血管生成及其對Wnt-β-catenin-VEGF信號通路的影響.pdf

1、學(xué)號密級埸H1大萼YANGZHOUUNIVERSITY碩士學(xué)位論文(學(xué)術(shù)型)GBEE抑制Lewis肺癌腫瘤血管生成及其對Wn邯一catenin—VEGF信號通路的影響指導(dǎo)教師姓名：申請學(xué)位級別：論文提交日期：學(xué)位授予單位：韓東冬碩士2Q12生壘旦量揚(yáng)劌盤鱟學(xué)科專業(yè)名稱：葒堡生論文答辯日期：2Q12生蔓月2量學(xué)位授予日期：2Q!Z生魚旦三Q量20l7年5月?lián)P州大學(xué)碩士學(xué)位論文2AbstractObiective：Studyon也eacti

2、Vi夠ofG勵(lì)最妒6洳6口eXocaq)eXtracts(GBEE)againstt哪orangiogenesisinvitroa工1dvivoanditsinfluenceonW州D—catellin—VEGF(VaLScularendothelialgro叭hfactor)signalpat11waysandPKB(proteinkinaseB)byculturingLewislullgcallcer(LLC)cellinVitro

3、a工1destablishingLLCmetastasismodelofC57BL／6Jmiceinvil∞teVealpartialmolecularmechanismofGBEEinhibitionofangiogenesisMethods：TheeffectofGBEEonproliferationofLLCcellswasdetectedbyMTTmethodinVitroThemiceLLCmetastaSismodelwas

4、setup，Thec57BL／6Jmice、vererandomlyseparatedintonorrnal，model，positiveandGB髓(50，100，200mg瓜g)treatmentgroups，n_10No吼a1groupdidnotreceivemmorceUsuspensionThemiceinnormalgroupaIldmodelgroupwerebothintragastriegavage(ig)no吼a1

5、saline(NS)inavoluIneof01mL／l0g(bw)，positiVegroupwasintraperitoneal(ip)i11jectioncyclophosph鋤ide(CPA)atadoseof20mg／kg(bw)，theGBEEtreatmentgroupswererespectiVelyigGBEE50，100，齜1d200mg／kg(bw)，onceadayfor20dARertreatment，weca

6、Iculated血etumorinhjbitionmte，lungmetastasisrateanda工1timetastasisrateHEstaillingwasusedtoobserVethehjstologyoflungandtransplantedt呦ortissuesThemicrovesseldensity(MVD)wasmeasuredbyimmunohistocheI工1istrymethodint啪spl趾tedtⅦ

7、norTheeXpreSsion1evelsofVEGF甜1dVEGFR2(VascularEndotheliaIGroⅢhFactorReceptor2)mRNAorWnt，pcateni工1，VEGF，vEGFR2andp—PⅪ3／PKBproteinexpressionwererespectiVelytestedbyqRT—PCRorwesternblotinVitroandViVoResults：GBEEsuppressedth

8、egrowthofLLCcellsinaconcentrati。n—dependentwayat(№concentrationsof5，10，20，40，80and160肛g／mLinvitroTheLLCcellsweretreatedwithGBEE(10、20、40pg／rnL)for48h，itcansignmcamlysuppressW11tandp—cateninproteinexpressiona11dthecomemof

9、mRNAofVEGFandVEGFR2inLLCcellsInViVo，wediscoveredGBEE(50，100，200mg瓜g)caJlsignificamlysuppresst11egrowmofC57BL／6JmiceLLCtransplarltedtumorandLLClungmetastasis，andreducetheexpressionofCD34，whichmeansMVDwasi11hibited，a11ofⅥ恤