芽孢桿菌纖溶酶的純化、性質(zhì)及其發(fā)酵條件的研究.pdf

1、西南農(nóng)業(yè)大學(xué)碩士學(xué)位論文芽孢桿菌纖溶酶的純化、性質(zhì)及其發(fā)酵條件的研究姓名：王光利申請學(xué)位級別：碩士專業(yè)：細(xì)胞生物學(xué)指導(dǎo)教師：李名揚楊星勇2001.5.1西甫農(nóng)業(yè)大掌碩士掌位論文一—————————————————————————————————————————————————————————一Purification，CharacterizatiOnandFermentationConditionsofFibrinolyticEnzym

2、efromBacillusspCanditate：WangGuangliAdvisor：ProfLiMingyangAProfYangXingyongProfPeiYan(BiotechnologyResearchCenterSouthwestAgriculturalUnivertity，Chougqing，400716)Withlivingconditionsimproved，thrombosisdiseaseshavebecomeg

3、reatthreateningtohumanbeingsThrombolytictherapyisoneofthebestwaystocurethrombosisdiseasesFibrinolyticenzymeshaveapparentsignificanceinthrombosistherapyinhumanAtpresent，thrombolyticagents(tPA)wastransformed，atthesametime，

4、therehasbeeninterestinsearchfornewthrombolyticagentsfromvariousoriginswithparticularreferencetomicrobialsourcesBacillussp2，whichproducesastronglyfibrinolyticenzyme，wasscreenedinourlaboratory，todeveloptheenzymeforUSeasath

5、romboticagentInthispaper，throughsinglefactorsandhomogenousdesignexperiment，thesuitableconditionsweregiventoproducefibrinolyticenzymefromBacillussp2(Bs一2FE)TheenzymewaspurifiedfromthefermentedbrothofBs一2andcharacterizedin

6、substratespecificity，inhibitorcharacters，optimumtemperatureandpHthermalstability，effectsofmetalionsTheresultsareasfollows：ThefermentationconditionsinshakingflaskswereinvestigatedBythemethodofhomogenousdesignexperiment，th