多毛番茄(Lycopersicon hirsutum)中抗冷轉(zhuǎn)錄因子LhCBF1基因的遺傳轉(zhuǎn)化.pdf

1、ClassifiedIndex:U.D.C:DissertationfortheMasterDegreeinAgricultureGENETICTRANSFORMATIONOFCRTDREBINDINGFACTORCBF1GENEINHAIRYTOMATO萬從SUTU叼夕必YCOPER國ICONCandidate:SuPervisor:AcademieDegreeAPPliedfor:SPeeiality:DateofOralExami

2、nation:University:ZhuW己n比eProf.MangAoxueMasterofAgricultureOlericultureJune.ZO10NortheastAgriculturalUniversity了東北農(nóng)業(yè)大學(xué)農(nóng)學(xué)碩士學(xué)位論文2.7.2農(nóng)桿菌的培養(yǎng)...................................................................................

3、......……302.7.3侵染和共培養(yǎng).............................................................................…………302.7.4外植體的篩選.........................................................................……~……302.7.5外植體的繼代.......…….....

4、.......................................................................................……312.7.6生根培養(yǎng)..................................................................................................................……312.

5、7.7馴化移栽.................................……:.…、............................……，.................................……312.8抗性植株的PcR檢測..................................................4……312.8.1番茄基因組DNA的提取......................

6、.................................................................……312.8.2引物的設(shè)計(jì)及PCR檢測.................................…………‘.........................................……322.9轉(zhuǎn)基因植株的耐低溫性檢測.....................................

7、....……，.........................................……332.9.1植物材料..................................................................................................................……332.9.2低溫脅迫誘導(dǎo)..............................

8、.......……:...................................……，..……332.10CAMV35S和RD29A啟動(dòng)子分別調(diào)控類番茄茄中CBFI基因的載體構(gòu)建..……，.……332.10.1類番茄茄中CBFI基因與pMD18一T載體的構(gòu)建.............................................……332.ro.2CaMV35S調(diào)控類番茄茄中CBFI基因的載體構(gòu)建......

9、...................................……332.ro3Rd29A啟動(dòng)子調(diào)控類番茄茄中CBFI基因的載體構(gòu)建..................................……342.ro.4pcAMhP2408CBFI和pRDhP2408CBFI載體轉(zhuǎn)化農(nóng)桿菌.........................……:.…343結(jié)果與分析.................................

10、....................................................................……:................……353.1低溫脅迫對多毛番茄幼苗生理生化特性的影響.........................................................……353.1.15℃低溫脅迫對多毛番茄幼苗MDA和02一含量的影響...............

11、......................……353.1.25℃低溫脅迫對多毛番茄幼苗SOD和POD活性的影響..................................……353.1.35℃低溫脅迫對多毛番茄幼苗脯氨酸含量和CAT活性的影響.........................……363.1.45℃低溫脅迫對多毛番茄幼苗ASA和PAL活性的影響..................................…

12、…373.1.55℃低溫脅迫對多毛番茄幼苗葉片的影響.........................................................……383.2多毛番茄中cBFI基因與PMD18一T載體的構(gòu)建........................................................……383.2.1多毛番茄中CBFI基因的PCR擴(kuò)增和回收.....................

13、...........……，..................……383.2.2菌落的PCR和酶切鑒定........................................................................................……393.3CAMV35S調(diào)控多毛番茄CBFI基因的PCAMHp1O33載體構(gòu)建.............................……403.4助29

14、A調(diào)控多毛番茄CBFI基因的pRDHP1033載體構(gòu)建.......……J........................……423.5植物表達(dá)載體PCAMHP1033和pRDHP1033轉(zhuǎn)化農(nóng)桿菌...........................................……433.6CAMV35s和助29A啟動(dòng)子分別調(diào)控類番茄茄中cBFI基因的載體構(gòu)建............……443.6.1CaMV35S調(diào)控類番茄茄CB

15、FI基因的pCAMhP2408載體構(gòu)建......................……443.6.2Rd29A調(diào)控類番茄茄CBFI基因的p盼hP2408載體構(gòu)建...............................……453.6.3pCAMhP2408CBFI和pRDhp2408CBFI載體轉(zhuǎn)化農(nóng)桿菌................................……453.7番茄的遺傳轉(zhuǎn)化...................

16、.........................................................................……463.8再生植株的PcR檢測.……，.............................................……473.9轉(zhuǎn)基因植株的耐低溫性檢測..............................................................