黑腹果蠅亮紅眼突變型的遺傳學(xué)和分子生物學(xué)特性分析.pdf

1、中國(guó)農(nóng)業(yè)大學(xué)博士學(xué)位論文黑腹果蠅亮紅眼突變型的遺傳學(xué)和分子生物學(xué)特性分析姓名：楊軍申請(qǐng)學(xué)位級(jí)別：博士專業(yè)：動(dòng)物遺傳育種與繁殖指導(dǎo)教師：連林生吳常信20060601中國(guó)農(nóng)業(yè)大學(xué)博二：學(xué)位論文AbstractAbstractAsanidealgeneticmaterialandmodelanimal，Drosophilamelanogasterhasbeenusedforgeneticresearchforabout100yearsComp

2、oundeyetraitssuchaseyeshapeandeyecolorarealwaysthehot—spotsforgeneticresearchThenormalred—browncompoundeyecolorofDrosophilamelanogasterisduetothepresenceoftwotypesofpigmentsThereddrosopterinissynthesizedfromguanineviapte

3、ridineintermediateswhilethebrownxanthommatinisderivedfromtryptophanviafourenzymaticreactionsThepigmentprecursorthentransportedintopigmentcellsbytransmembranetransportersEyecolormutantswithbright—redeyeWasfoundinF2crosspr

4、ogenyofBandprfliesAfterwildtypefliescrossedtoBandprfliesrespectivelymutantbright—redeyeflieswereobtainedinF2progenyofwildtypeandBfliesbutnoeyecolormutantswasfoundincrossprogenyofwildtypeandprflieseveninF7progenyPurebrigh

5、t—redeyeflylineWasobtainedbyinbreedingThemutantgeneresponsibleforthebright—redeyewasidentifiedasanalleleofscarletgenewhichlocatesonchromosome3afterthebright—redeyefliesWascrossedwithtypeflies，underwenttwo—pointtestswithv

6、gandemutantsandcomplementationtestswitheyecolormutantscn。，st。andV。respectivelyThebrightredeyemutantflylinewasnamedass亡rEyepigmentofwildtype，B，stb7andst。fliesWasextractedandtheabsorbanceWasmeasuredEyepigmentcontentofdiffe

7、rentflylinesWasanalyzedbysignificancetestThexanthommatincontentdifferencebetweenwildtypeandeyecolormutantsstb7andst。isextremelysignificant(JP005)NosignificantdifferenceofxanthommatincontentWasfoundbetween5，andst。flies(P0

8、05)Bflieshavemuchmorexanthommatinthans產(chǎn)randst／flies‘P如01)butsignificantlylessthanwildtypeflies(POO1)ItisconcludedthatlowlevelofxanthommatincontentinthecompoundeyeisresponsibleforthemutantbrightredeyephenotypeSixpaimofpri

9、mersweredesignedforamplifyingscarletgeneA75一kb412elementinsertionwithinexon4ofscarletgeneWasdetectedinB，spandstiflieswiththefourthpairprimersThe412elementinsertedinto1612‘“bp～1613mbpofst。scarletgenewitha450bplossof5“LTRi

10、nthesamedirectiontosc口mftranscriptionwhilethe412elementinsertedinto1722ndbp1723阿bpofs，scarletgeneinthereversedirectiontoscarlettranscriptionRTPCRforexonsamplificationofdifferentflylinesWasperformedSevenexonswithnosequenc

11、ealterationwereamplifiedinwildtypeandBfliesandexons13withnosequencealterationwereamplifiedinspandstlfliesTherewerenocDNAproductsamplifiedwhichiscorrespondenttothesequencebetweenexon4‘“to7mKeywords：Drosophilamelanogaster，