無(wú)膠篩分毛細(xì)管電泳相互作用分析及毛細(xì)管電泳手性拆分.pdf

1、重慶大學(xué)碩士學(xué)位論文無(wú)膠篩分毛細(xì)管電泳相互作用分析及毛細(xì)管電泳手性拆分姓名：黃芳申請(qǐng)學(xué)位級(jí)別：碩士專(zhuān)業(yè)：分析化學(xué)指導(dǎo)教師：夏之寧20040430重慶大學(xué)碩士學(xué)位論文英文摘要ABSTRACTThepaperismadeupoftwoparts，thefirstpartisnongelcapillaryelectrophoresisinteractionanalysisSincemigrationtimewindowistoosmall，c

2、apillaryzoneelectrophoresispeakshiftmodelCan’texactlymeasuretheinteractionbetweendrugandproteinwhichhavesimilarmobilitiesThemechanismofmolecularsievingofentangledpolymersolutioninnongelcapillaryelectrophoresismGCE)wascit

3、edinthepaperInordertoexpandmigrationtimewindowentangledpolymerwasusedtochangethemobilityofproteininthebuffersolutionThemodelofinteractionanalysisbetweenproteinandligandpossingsimilarmobilitieswasestablishedbyNGCEThismeth

4、odfllrtherdevelopedtheanalyticalmethodsofcapillaryelectrophoresisinteractionanalysis1SincemobilityratioisindependentofviscositiesitWasintroducedtothecalculationmodelofcapillaryelectrophoresisinteractionanalysis，inorderto

5、efficientlyeliminatetheeffectofviscositiesofentangledpolymersolution2Linerpolyacrylamidewasusedassievingpolymertodeterminethebindingconstantbetweenchlorpheniramineandbovineserurualbumin(BSA)IncontrastwithNGCE，capillaryzo

6、neelectro。phoresisWasalsousedtomeasurebindingconstantbetweencMorpheniramineandBSATheresultsoftwomethodswerealmostconsistent3DextranwasusedassievingpolymertodeterminethebindingconstantsbetweenIndomethaein，andBSA，Irbesarta

7、nandBSAseparatelywhichweresimilarwithmobilitiesThesecondpartisehiralseparationofcapillaryelectrophoresisTheenantiomericseparationsofchlorpheniramineandsibutraminebycapillaryelectrophoresisusingp—cyclodextrin(p—CD)astheeh

8、iralselectorwerestudied1Bindingconstantsbetweentheenanfiomersofchlorpheniramineandp—CDwerestudiedatdifferentpHTherelationshipofresolutionandbindingconstantsWasdiscussedSincetheconcentrationofBCDatwhichthediscriminationof

9、enantiomericmobilitiesWasmaximalwasinconsistentwiththeconcentrationof13CDatwhichtheresolutionoftwoenantiomersWasoptimal，thepaperdiscoveredtherelationshipofresolutionandthemobilityoftwoenantiomers2Theenantiomericseparatio