轉(zhuǎn)抗菌肽PR39基因小鼠的制備及研究.pdf

1、I分類號(hào)：S482.39密級(jí)：碩士學(xué)位論文轉(zhuǎn)抗菌肽PR39基因小鼠的制備及研究董銳第一指導(dǎo)教師：第一指導(dǎo)教師：吳珍芳教授第二指導(dǎo)教師：第二指導(dǎo)教師：李紫聰副教授學(xué)院名學(xué)院名稱：動(dòng)物科學(xué)學(xué)院專業(yè)學(xué)位類別：專業(yè)學(xué)位類別：農(nóng)業(yè)推廣碩士領(lǐng)域：域：動(dòng)物遺傳育種與繁殖答辯委員會(huì)主席：答辯委員會(huì)主席：宋長(zhǎng)緒研究員中國(guó)廣州2016年6月IIGenerationStudyofPR39TransgenicMiceRuiDong(CollegeofAnima

2、lScienceSouthChinaAgriculturalUniversityGuangzhou510642China)Abstract：Antimicrobialpeptides(AMPs)playanimptantroleinanimal’sinnateimmunesystem.AMPshavebroadspectrumantipathogenicmicoganismsanticancereffects.Theyarerarely

3、toinducedrugresistancefmicganismseasytobedegradedinanimalbody.TherefeAMPsarewidelyconsideredastheidealalternativefantibiotics.AMPshavebeenusedasfeedadditivesinanimalhusby.HowevertheactivityofAMPsiseasytobedecreasedduring

4、theproductionstageshippingofAMPs.FurthermeadditionofAMPstofeedincrasesthecostoffarmanimalproduction.TheaboveproblemscanbesolvedbytransgenicexpressionofAMPsinanimals.InthisstudyavectcalledpCMSEGFPPR39wasconstructed.Thisve

5、ctcarriesapigderivedAMPgenecalledPR39(prolineargininerich39aminoacidpeptide).ThisvectcanexpressantibacterialPR39incultured293cells.EighttransgenicmicewereproducedbypronuclearmicroinjectionoflinearizedpCMSEGFPPR39plasinto

6、mousefertilizatedeggs.TheexpressioninheritanceofPR39transgeneintransgenicmicewereanalyzed.TheantibacterialabilitywascomparedbetweentransgenicmicetheirwildtypelittermatesfollowingartificialinfectionofswineActinobacilluspl

7、europneumonia(APP).Themainresultsareasfollws.(1)ThePR39transgenewasexpressedinvarioustissuesoftransgenicmicestablypassedtonextgeneration.Transgenicmicehaveasignificantlyhighergowthratethantheirwildtypelittermates.(2)Duri

8、ng524hpostinfectionofAPPthemtalityrateoftransgenicmicewassignificantlylowerthanthatoftheirwildtypelittermates.(3)At5hpostinfectionofAPPlivetransgenicmicecarrysignificantlylowernumberofAPPinvarioustissuesthanthatoftheirli