轉(zhuǎn)PPase基因馬鈴薯塊莖休眠和發(fā)芽特性的分析與評價.pdf

1、甘肅農(nóng)業(yè)大學碩士學位論文轉(zhuǎn)PPase基因馬鈴薯塊莖休眠和發(fā)芽特性的分析與評價姓名：張春鳳申請學位級別：碩士專業(yè)：作物遺傳育種指導教師：司懷軍20090601甘肅農(nóng)業(yè)大學2009屆碩士學位論文Abstract1f1圮characteristicsofpotatotuberdormancyandsproutingcouldbecontrolledbyregulatingCO—substrate(inorganicpyrophosphatas

2、e)inthesugarmetabolismInordertoobtainnewtransgenicpotatogermplasmwimprolongedorshorteneddormancyperiod，thephysiologicalandbiochemicalindexesweremeasuredandtuberdormancyandsproutingcharacteristicswereanalyzedandevaluatedf

3、ortransgenicPPasegenepotatoesThemainresultsasfollowing：1Economicalcharacteristicsofthetransgenicpotatoplantsinthegreenhouseweremeasured，theresultsshowedthattheplantheightWasincreased，andthetubernumberwasdecreasedforthese

4、nsetransgenicplantsofpotatocultivaGannongshu2，nleplantheightwasdecreased，thetubernumberWaSincreased，andtuberweightWasdecreasedfortheantisensetransgenicplantswhichcomparedwimtheuntransgenicplantsThereWasnosignificantdiffe

5、renceforthesensetransgenicpotatoplantsofpotatoeultivaFavoritacomparedwiththeuntransgenicplants，howerer，thereWassignificantdifferencebetweenthesensetransgenicpotatoplantsandtheuntransgenicplantsforplantheight2PPaseactivit

6、yandPicontentweremeasuredinthetransgenicandthewildpotatoplants，theresultsshowedthatforthetransgenicplantsofpotatocultivarsGannongshu2andFavoritawhichweredrivenbyconstitutiveexpressionpromoterCaMV35S，thePPaseactivityandPi

7、contentintheleaves，stemsandgrowingtubersofthesensetransgenicplantswereimproved，andtherewerereducedintheantisensetransgenicplantscomparedwimtheuntransgenicplantsForthetransgenicplantsdrivenbythelowtemperature—inducedexpre

8、ssionpromoterrd29A，PPaseactivityandPicontentveriedslightlyintheleaves，stemsandgrowingtubers，butthereWasasignificantlydifferenceforthemicrotubersstoredat4“Cand25“Ccomparedwiththeuntransgenicplants3Thecontentofstarchandsol

9、ublesugarwereassayedinthetransgenicanduntransgenicminitubersunderdifferentstoragecondition，theresultsdemonstratedthatforthetransgenicplantsdrivenbyCaMV35SpromoterstarchcontentWasnotvariedinthesensetransgenicminitubers，wh

10、ilesugarcontentWasincreasedsignificantlyandstarchandsugarcontentWasdecreasedintheantisensetransgenicminituberscomparedwiththeuntransgenicminitubersForthetransgenicplantsdrivenbyrd29Apromoterstarchcontentinthegrowingminit