蘇云金芽孢桿菌高效菌株的選育、發(fā)酵培養(yǎng)與添加劑篩選.pdf

1、福建農(nóng)林大學(xué)碩士學(xué)位論文蘇云金芽孢桿菌高效菌株的選育、發(fā)酵培養(yǎng)與添加劑篩選姓名：邱思鑫申請(qǐng)學(xué)位級(jí)別：碩士專(zhuān)業(yè)：植物病理學(xué)指導(dǎo)教師：關(guān)雄2001.5.1福建農(nóng)林大學(xué)碩士學(xué)位論文摘要AbstractThisstudymainlyconcernsthecontrolofvegetable’Simportantlepidopteranpest(Plutellaxylostella，Spodopteralitura，Spodopteraexigu

2、a)ofsouthchinaEliminatingplasmidsofBtstrains(WB2，WB3，WB6，8010)byhightemperaturetreatmentorbycomplextreatmentwithSDSandhi【ghtemperature，ahighlytoxicBacillusthuringiensisTSl6wasobtainedTheelectrophoresisprofilesofplasmidDN

3、Aindicatedthattheplasmidwhichislessthan2Kbwaseliminatedandtheprofileofplasmidof66KbbecamethinTheinsecticidalactivityofTSl6toPlutellaxylostellawasnoticeablyhigher(15foldincrease)thanthatoftheoriginalstrainWB6MediawhichWas

4、mainlycomposedofdextroseandpeptonewerescreenedwithshakeflaskfermentationandorthogonaltestResetsindicatedthatsporenumbersweremainlyinfluencedbycarbonandnitrogeninsteadofinorganicsaltAmonginorganicsalts，theinfluenceofFeS04

5、7H20ismorenoticeablethanothersSocompositionofthemediumwasdividedintothreefactorscarbonsources(x1)，nitrogenSOurces(X2)andinorganicsaltsWithfixedinorganicsaltscontent，therelationshipbetweenfermentationmediawithdifferentcar

6、bonandnitrogensol／rcesandsporenumbersWasdemonstratedbyusingthedoublefactorsOrthogonal—rotationcombinationtestAresponseplaneequationwasformedasfollows：y=15520013380x：22866x224097x1222682x2Z17575xlx2It’seasytOacquirethebes

7、tcombinatorialmediumfromthisequationBacillusthuringiensiswasgrowninCulturemediainwhichdifferentagriculturalbyproductsasthemaincarbonandnitrogensourcesTheseproductsincludedCOrnmeal，starchdefaRedsoyfoUfishmeal，defa垃edpeanu

8、tflourandyeastsOfthisseveralproductstested，fishmealanddefattedpeanutflourwerethemostefficientsubstratesforBtWithorthogonalexperiment，Agoodcombinatorialmedium(defaRedsoyfour32∥L，cortameal14g，L，defaRedpeanutflour32g／L，fish