細(xì)胞壁鈣庫在棉花細(xì)胞氧化迸發(fā)中的作用.pdf

1、華中科技大學(xué)碩士學(xué)位論文細(xì)胞壁鈣庫在棉花細(xì)胞氧化迸發(fā)中的作用姓名：張一竹申請學(xué)位級別：碩士專業(yè)：生物化工指導(dǎo)教師：劉曼西2003.5.1華中科技大學(xué)碩士學(xué)位論文AbstractVerticiliumdahliaeklebandFusariummoniliformeSheldaretwopathogenicfunzithatcausecottondiseasesStudyingtheroleofcellwallcalciumduringt

2、heoxidativeburstwillconducemuchtostudythesignaluansductionpathwayduringplantdefenseresponseByusingConfocalLaserScanMicID∞ope(CLSM)inconjunctionwithH202reporterdyeDCFHDAoxidativeburstincottonguardcellscouldbedetectedwhent

3、reatedwithpectase，thoughnotaspromptlyastheoxidativeburstcausedbyV44elicitorThisresultsuggeststhatpectasehydrolysispectinintheplantcellwallandthefragmentsactasinternalelicitorstocausedefenseresponse硼nlleoxidativeburstinco

4、ttonguardcellscouldnotbedetectedwhentreatedwithpectaseinabsenceofextracellularCa2ByusingcalciumreporterdyeFura2AM和Fura2，thecytosolic【ca2】and[cQqinthecellwallinguardcellsismeasuredafterthetreamlentofpectaseinMESbufferwher

5、eCa2isremovedTheresultshowsthatpectasecouldnotcauseacytosolic【calincreaseorfree[Ca2】increasefromthecellwallinabsenceofextracellularCa2InabsenceofextracellularCazoxidativeburstinguardcellscouldbeobservedwhentreatedwimelic

6、itorsfromVerticiliumdahliaekleb(V44elicitor)orFusariummonilifoFllleSheldin3rain，whilethereisnooxidativeburstwhentreatedwithyeastoligosaccharinsTheseresultsfurtheldemonstratethatoxidativeburstisadefenseresponseinplantThee

7、licitorsreleasebindingcalciumfromthecellwallandcauseoxidativeburstduringthecottonpathogeninteractionsWealsostudytheeffectof1mmol／Lsalicylicacid(SAloncottonguardcellsinabsenceofextracellularCa2andfinditcouldnotcauseoxidat