31246.sertoli細(xì)胞吞噬凋亡生精細(xì)胞的機(jī)理和意義

1、北京協(xié)和醫(yī)學(xué)院中國醫(yī)學(xué)科學(xué)院博士學(xué)位論文Sertoli細(xì)胞吞噬凋亡生精細(xì)胞的機(jī)理和意義姓名：熊偉鵬申請(qǐng)學(xué)位級(jí)別：博士專業(yè)：細(xì)胞生物學(xué)指導(dǎo)教師：韓代書陳實(shí)平20090501北京協(xié)和醫(yī)學(xué)院博上研究生學(xué)位論文AbstractAbout75％ofspermatogeniccellsareestimatedtoundergoapoptosisduringmammalianspermatogenesis，andthecytoplasmicporti

2、onsoftherestgermcellsareshedandformresidualbodiesbeforetheymatureintospermatozoaTheapoptoticspermatogeniccellsandresidualbodiesarephagocytosedanddegradedbySertolicellsThemechanismsandsignificanceunderlyingthesephenomenar

3、emaintobeclarifiedInthisstudyweinvestigatetheseissueswithaneffortontheessentialroleofTyr03receptortyrosinekinasesubfamilyinregulmingphagocyticabilityofSertolicells，andthemeaningofphagocytosisofapoptoticgermcellbySertolic

4、ellsWeestablishedaninvitromethodtoevaluatethephagocyticabilityofSertolicellsthroughdetectinglipiddropletformationByusingthismodel，weobservedt11atTyr03subfamilyofreceptortyrosinekinases(RTKs)——Tyr03，Axl，MerandtheirligandG

5、as6regulatephagocyticabilityofSertolicellsThepresenceofGas6inserumfreemediumincreasedphagocyticabilityofSertolicellsby5timesByanalysisoftheTyr03RTKsdeficientSertolicells，wedemonstratedthatthethreemembersofTyr03RTKsactcoo

6、perativelyinregulatingthephagocyticabilityofSertolicellsMerisresponsiblefortriggeringphagocytosisofapoptoticspermatogeniccellsbySertolicells，Tyr03andAxlenhancecooperativelytheactionofMerbyrecognizingandbindingapoptoticce

7、llsFurthermore，wedemonstratedthatapoptoticspermatogeniccellsandresidualbodiesCanbeenergysourcesforSertolicells／nvitroassayshowedthatengulfmentofapoptoticspermatogeniccellsincreasesATPproductionbySertolicellsTheATPproduct

8、ioninseminiferoustubulesiscloselyrelatedtothephagocytosisofresidualbodiesbySertolicellsInducedapoptosisofspermatogeniccellsinvivoincreasedATPlevelinseminiferoustubulesTheATPproductionbothinvivoandinvitroassociatedwiththe

9、lipidformationinSertolicellsafterphagocytosisofapoptoticspermatogeniccellsWethereforeconcludethatafterphagocytosisbySertolicells，apoptoticspermatogeniccellsaredegradedtoformlipids，whichcanbeusedtoproduceATPTheresultsdefi

10、neanovelmeaningofspermatogeniccelldeathFinally，wefoundthatlysatesofapoptoticgermcellsCantriggerinflammatoryresponsesbySertolicellsThelysatesinducedexpressionofvariousinflammatorycytokinesinSertolicellsThisprocessdependso