16685.負(fù)調(diào)控因子psatf2的功能及其相互作用蛋白的分離鑒定

1、版權(quán)聲明任何收存和保管本論文各種版本的單位和個人，未經(jīng)本論文作者同意，不得將本論文轉(zhuǎn)借他人，亦不得隨意復(fù)制、抄錄、拍照或以任何方式傳播。否則，弓起有礙作者著作權(quán)之問題，將可能承擔(dān)法律責(zé)任。AbstractTheFunctionoftheNegatiVeRegulationFactorPsATF2andthelsolationofitsInteractingProteinsAstherate—limitingenzymeofthephen

2、ylpropanoidpathway，chalconesynthaseplaysanimponantroleinplantdefenceWhenplantsareinfectedbypathogens，thechalconesynthasegenesrelatingtodefencemechanismarein(1ucedandhighlyexpressedOurpreviousworkhadidenti6edatrans—acting

3、factor尸s彳7，52(2西“，”苧口，^，“聊ATnchbindingfactor2)whichmayinVolVeintheregulationof風(fēng)(HS，byyeastonehybridscreensystemusingaJP西甜所s口f知“聊cDNAlibra叫inducedbyGSHInordertostudytheroleoftheEARt)rpetrans—actingfactorP叫刀堙intheplantdefe

4、ncemechanismregulatedbyPsCHSl，weused彳陽6f岫西歷口，f冊口asplantmate^alsandfcundPsATF2一oVerexpressiontransgenic么ra6lc幻ps括砌口髓D刀口showingphenotypeslikegrowthretardation，curledleaVes，PCD，enhanceddiseasesusceptibilityandpollensterilit

5、yFurthermore，thereal—timeqPCRresultsshowedthatoVerexpressedPsATF2supressedtheexpressionofCHSgenefamilym鋤bersof彳r臼6蚴括，辦口?？?，口：77￥，上彳P5and以P6comparingtothewildtypeToexplorethepossiblemechanismcausingthosephenotypes，weidenti

6、fied觚oPsATF2interactingproteins，A24andA25，byyeasttwohybridscreenandpreliminaryproVedtheinteractionsofA24andA25withPsATF2111summary，thisstudyreVealsmephenomenonofthe彳陽6j卻s西CHSgenefamilymembersaresuppressedbytheoVerexpress

7、ionof尸扭“明s口髭V“班仃ans—actingfactor剛7衛(wèi)andidentifiedtwointeractingproteinsofPsATF2，A24andA25WeassumeA24andA25mayresponsetotheupstreamcalciumsignalsofplantdefence，interactwithPsATF2andregulatetheexpressionofCHSgenesKeyWords：C