15311.利用生物磁化水培養(yǎng)蛹蟲草及對(duì)其活性物質(zhì)含量的影響

1、Univers塒Code：l0225RegisterCode：DissertationfortheDegreeofMasterUsingMagnetizedWraterCultiVationofCD砂c刪mfZi砌r括andtheEfrectonBioactiveMetaboliteProductionCandidate：SuperVisor：AssociateSuperVisor：AcademicDegreeAppliedfor：Sp

2、ecia時(shí)：DateofOralExamination：Universi“：ZhaoBoZhangGuocaiMasterForestProtectionNonheastForest巧UniVersityAbstractCo耐弦PpJ^糾豇口廠西，ⅥmichismetypespeciesofCD吻c：夠矽，isoneofthemoStiInponantmretraditionalChineseherbalmedicinesNowaday

3、s，aspeopIe’srecognitionofthehealtllcaremnctionofCD朋弦Pps^衙，if訂r括，theimproVingofthequalityrequirements，，the’m孤ketdemandcontinuesincreasingandthedeepeningoftheresearchaIlddeVelopment，tllescaleo士cultivationhasalsobeenexparld

4、ingBut，foralongtime，dueto“sVolatileyieldandqualityandseriousdegradation，whichrestrictsthedevelopmentoftheindustryof(b，矗pc已ps^衙，ffc礦捃Thisstudvwasonthebasisofconventionalculturetechniques，whichmagnetizedwaterwasusedtoconfe

5、cttheculturemedi眥tocultureCo，砂cepsml，f砌r括，significannyincreaSeditsproduction，cordycepin，cordycepsacidaIldpoIysaccharidecontentTheresultswereaSfollows：(1)UsingmagnetizedwatertoculturethestockseedofCordycepsmilitaris，which

6、waStreatedfor9timesonordinarywatefwith6eIdstrengthof010T、O25T、040T’whiletreatingmeseriesconnection(SC)3times，innowvelocityfor10m。s—andmedistanceof30cm，respectivelyThecolonygrowthratehaVebeenimpmVedinalltreatmentsInO4Ttre

7、atment，thecolonygrowchratewas367mm。d1，whichincreased538％comparedwlth也econtrast，istheoptimummagnetized、vaterforstockseedcultiVation(2)Duringthe1iquidculturepmcess，usingthe01Tmagnetized、vatercallslgnltIcantlyimprovetheanly

8、Iaseactivi夠，thehighestactiVitywaS448U，3494％highercomparedwnhmecontrastHowevekthe04TmagnetizedwatercansignificantlyimproVetheactiVit)，otpolyphenoloxidaseandprotease，advancethetimeofproteaseactiVitypeaksimultaneouslyInthe0

9、4Ttreatment，thepolyphenoloxidaseactivitywas230U，whichincreased1675％comparedwi也t11econtrast，theproteaSeactivit)，wasupto681U，3898％higherthanmecontrastAttllesanletime，duringtheliquidculturestage，therestofmagnetlZedwaterc明al

10、soDromotetIlesecretionofthethreeextracellularen巧mes，andincrease1nltsactlVItyconesponding(3)usingdi療erentmagneticfieldstrengthcultureofCD咖EPJ所f，f，口，西，canincreasetllea11dtreatedmethodsofmagnetizedwaterliquidd巧weightofmycel

11、ium，cordycepin，cordycepsacidandpolysaccharidecontemTheO4T仃eatmenthasthehighestmycelialdrywelghtarldcordycepsacidcontentof150g100IIlL‘1肌d6932mgg～，increased2712％arId2293％comparedwitIlthecontr嬲t，respectivelyThe025Ttreatment

12、w2LsfaVorableforcordycepmproductionofCD曲c緲朋訂i柵括，whichmecontentwasupto332mg’91，149％hi曲er也anthecontraStIn01Ttreannenttrea_nIlenthastllehighestmyceli啪polysaccharidecontentol3630mg／g，whichincreasedl832％cOmpared、Ⅳitllthecontr