環(huán)氧化酶及其抑制劑與宮頸癌關系的研究.pdf

1、山西醫(yī)科大學碩士學位論文環(huán)氧化酶及其抑制劑與宮頸癌關系的研究姓名：畢慧玲申請學位級別：碩士專業(yè)：婦產(chǎn)科學指導教師：田秀珠20050516山西醫(yī)科大學硬士學位論文Abstractobject：(1)ToinvestigatetheexpressionofCOX一1andCOX2incervicalcarcinoma，CINandnormalcervicalepitheliumToevaluatethecorrelationbetweent

2、heCOXsandelinico—pathologicvariablesinthesecervicalcarcinomapatients(2)ToinvestigatetherelationshipbetweenCOX一2andKi一67proliferationindexs，bax，microvasculardensity(MVD)andCD44v6respectively(3)Tostudytheinhibitoryeffectso

3、fcelecoxibaselectivecyclooxygenase2inhibitorandaspirinanonselectivecyclooxygenaseinhibitorontwocervicalcarcinomacell】inesinvitroMethods：(1)TheexpressionofCOX一1，COX2，wereexaminedbyimmunohistochemistryPVmethodandtissuechip

4、methodin101casesofcervicalcarcinomas12casesofCINand10casesofnormalcervicalepithelium；Ki一67，bax，MVD(1abelledbyCD34)andCD44v6wereexaminedinl01casesofcervicalcarcinomasattheSametime(2)111eeffectofcellcycleandcellapoptoticra

5、teswereanalyzedbyflowcytometry(FCM)Results：(1)111epositiveexpressionratesofCOX一1havenosignificantdifferencebetweencervicalcarcinomas，CINandnormaleervicalepitheliumCOX2proteinCannotbedetectedinnormalcervicalepithelium；111

6、epositiveexpressionratesofCOX2inCIN(667％)andcervicalcarcinomas(871％)weresignificantlyhigherthaninnormalcervicalepithelium(P005)(2)TheexpressionofCOX1incervicalcarcinomashasnocorrelationwimage，clinicalstages，grades，invasi

7、ondeepandmetastasisoflymphaticnode；TheexpressionofCOX2incervicalcarcinomashasnocorrelationwitllageclinicalstages，gradesandinvasiondeep，butithasacorrelationwitllmetastasisoflymphaticnode(p005)(3)TheexpressionofCOX2incervi