預(yù)照射聯(lián)合siRNA抑制小鼠肺癌survivin基因表達的研究.pdf

1、分類號： R73-3 密級：UDC： 610 學(xué)校代碼：11065碩士學(xué)位論文預(yù)照射聯(lián)合 siRNA 抑制小鼠肺癌 預(yù)照射聯(lián)合 siRNA 抑制小鼠肺癌 survivin 基因表達的研究 survivin 基因表達的研究王 利 華 王 利 華指 導(dǎo) 教 師 閻超 副教授學(xué)科專業(yè)名稱 腫瘤學(xué)論文答辯日期 2015-06-03Research on the expression of survivin gene suppressed by

2、pre－irradiation and intratumor injection siRNA in lung cancer of mouse.AbstractObjective: This study was to explore the effects of the expression of survivin genesuppressed by intratumor injection siRNA and radiation in

3、lung cancer.Method: Mouse subcutaneous transplanted with tumors were randomly divided intofour groups: untreated group (groupA)、 group with intratumor injection siRNA (groupB)、group with radiation(groupC)、group 4Gy pre-i

4、rradiation combination with intratumorinjection siRNA (group D).Two days later, mouse with different treatments were executedby cervical dislocation and stripped the subcutaneous tumors. mRNA and protein levelsof survivi

5、n gene were detected by rquantitative Real-time PCR（qRT-PCR) and Westernblot；Cell cycle and cell apoptosis were assayed by flow cytometry (FCM).Result: The expression of survivin gene in mRNA and protein levels in group

6、B、 C、D was significantly reduced compared with group A. The differences of survivinexpression in mRNA level between group A and group D were statistically significant.Compared with another three groups, the expression of

7、 survivin gene in group D wassignificantly reduced, the differences were statistically significant （ P=0.036 ） ； theproportion of cells in S-phase of group D was 2.70±0.34%, compared with group B(8.93±0.75%) an

8、d group C (6.71±0.51%)，that was significantly reduced.The differencesof the proportion of S-phase cells in group A and group D were statistically significant；cell apoptosis in group D was 25.67±0.65%,which was

9、significantly increased comparedwith the cell apoptosis in other three groups ,variance analysis showed F =78.82, P<0.05,the differences were statistically significant.Conclusion: Pre-irradiation can enhance the trans