乳腺癌侵襲與轉(zhuǎn)移相關(guān)基因差異性表達(dá)的研究.pdf

1、南開(kāi)大學(xué)碩士學(xué)位論文乳腺癌侵襲與轉(zhuǎn)移相關(guān)基因差異性表達(dá)的研究姓名：敬銳申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：臨床醫(yī)學(xué)指導(dǎo)教師：孫慧20040525塑莖查堂墮蘭墮堡蘭蘭墮望莖一一』墮!!!旦ABSTRACTObjective：Toobservethedifferentialexpressionofre]ativedgenesjnthemetastaticprocessofbreastcanceronthebaseofanimalmodelBCML—TA

2、299，discussthepossiblemechanism，andtryteestahlishanewmimaImedelwithMgherlungmetastaticrateMethods：DividedtheexperimentalriceintotwogroupsrandomlywhichwerecalledGrouplandGroup2，transp]atedthetumortissuefromtheoriginaltumo

3、rfocusofI，ulnogloadedmouseofBCMITA299tothebreastregionofGroup1mice，aridtransp]atedthetumortissuefromthelungmetastatictumorfocusoftumorloadedmouseofBCMLTA299tothebreastregionof“Group2mice，observedthegrossForm、patho]ogicfo

4、rmandchromosomeformofthetumors，measuredtheexpressive1evelofP16，Survivin，NrasandCmycbythetechnoiOgyofinmlunohistochemistryandcomparedthedifferentiatexpressionof“thegenesbetweentheoNginalfocusandmetastaticfocusResults：1)Co

5、mparedwiththemiceofGroup1，themiceofGroup2didn’tshowanystaristicsSjgnificantdifferenceintherateofmetastasiS(PO05)；2)ComparedwjththemiceofGroup1，themiceofGroup2didn’tshowanydifferenceinthefie]doftumormorpho]egy，bothofthemw

6、erediagnosedBreastCancerTypeA；3)ComparedwiththemiceofGroup1，themiceofGroup2didn’tshowanydifferenceintheform。j1tumorchromosome，bothofthemwerespecialtumorchromosomesofmice：4)P16，SurviVin，NrasandCmycshowstrongexpressionwhea

7、therintheGroup1orinGroup2，weatherorigina]fOCUSormetastatiCfocus；5)ComparedWiththemiceofGroup1，themiceofGroup2didn’tshowanystarisLiessignificantdifferenceintheexpressivelevelofP16NrasandC—mycwhetherintheorigihalfocusorint