順鉑白芨混合微球誘導(dǎo)大鼠肝癌細(xì)胞凋亡的實(shí)驗(yàn)研究.pdf

1、Y957932學(xué)院代號(hào)專業(yè)代碼學(xué)號(hào)密級(jí)陜西中醫(yī)學(xué)院ShaanxiUniversityofChineseMedicine碩士學(xué)位論文順鉑白芨混合微球誘導(dǎo)大鼠肝癌細(xì)胞凋亡的實(shí)驗(yàn)研究學(xué)位申請(qǐng)人專業(yè)名稱申請(qǐng)學(xué)位類型李紅曉中西醫(yī)結(jié)合臨床外科專業(yè)學(xué)位指導(dǎo)教師姓名王治全教授主任醫(yī)師論文提交19期2006年4月TheresearchontheapoptosisofhepatomaoeIIsinducedwithcispIatinsbIetiIIamix

2、edmiorospheresAbstractIObjeetivel：Toinvestigatetheeffectsandmechanismofmixedmicrospheresontheapoptosisinhepatomacells，andexplorethevalueofitsapoptosisinducedbymixedcisplatinsandbletillamicrospheres【Methods]：fortymousewer

3、erandomlydividedintofourgroups(controlgroup，mixedbletiUamicrospheresgroup125][group，cisplatinsgroup)，and16foreachgroupChromationcondensationwasobservedbyCOstainingwithfluorescentdyesApoptotissubG1wasdetectedbyflowcytomet

4、ryandDNAladderWaSobservedusingagarosegelelectrophoresisIResultsl：BletillaincombinationwithcisplatinWasfoundtohave3ynergeticeffectsontheproliferationofhepatomacellsnledatashowedthathepatomacellstreated謝t11bletillaandcispl

5、atinincombinationproducedinternucleosomalDNAflagmentationanalysedbyagarosexlactrophoresisTl倫resultsofflowcytometryshowedthatbletillaandcisplatinadministratedincombinationshowednoobviouschangeinG1phasedistributioncompared

6、withsingletreatmentHoweverthiscombinationreducedtheSphasearrestandreversedthereductionofG2／MphaseinducedbycisplatintreatmentTheresultsalSOshowedthatthereWas113％or937％ofcellsundergoingapoptoticcellsinhepatomacellstreatedb

7、ycisplatinrespectivelywhileitshowed324％ofapoptoticcellsincombinationtreatmentCisplatin“3IandmixedmicrospheresWaSshowntoinducetypicalc11romatincondensationinhepatomacells【Conclusion]：Theresultssuggestthatbletillaenhanceme

8、ntofcisplatininducedapoptosisassociateswithdecreaseofproteinexpressionwhichmaybeUsefulforcanceerchemotherapy【Kcywords]：cisplatinsbletillamixedmicrospheres(CBMM)；cellapotosis；hepatoma；CI’splatinsAuthor：LihongxiaoTutor：Wan