熒光原位雜交在尿路上皮癌診斷中的應(yīng)用.pdf

1、天津醫(yī)科大學(xué)碩士學(xué)位論文熒光原位雜交在尿路上皮癌診斷中的應(yīng)用姓名：李晨光申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：外科學(xué)泌尿外指導(dǎo)教師：孫光20090501天津醫(yī)科大學(xué)碩士學(xué)位論文AbstractObjective：ToestablishtheexperimentalplatformofFluorescence／nsituhybridization(FiSH)indetectingurothelialcarcinomaDetectthechromosom

2、eabnormalitiesintheurinecellsofpatientswithurothelialcarcinomaTodiscusstheValueofFISH嬲anoninvasivediagnosismethodforurothelialcarcinomaMethods：，Urinesamplesofbothsuspectedurothelialcarcinomapatientsandhealthycontrolswere

3、collected，uril地cytologyandFISHwe∞synchronouslycheckedFISHwereusedtodetecttheabnormalitiesofchomosome37，17and9p210160100suspectedpatientsofurothelialcarcinomaand20healthycontrolsWeleenrolledinthisexperimentDataofthehealth

4、ygroupwerecollectedtoestablishthethresholdandpatientsofcarcinomaWCl“ediagnosedbythisthresholdThroughthecomparisonandstatisticalanalysisbetweentheresultsoftheexperimentsandpathologyweconcludedthesensitivityandspecificityo

5、fthetwomethods，aswellthedifferencebetweenhighgradeandlowgradecarcinomasResults：Urinesamplesofpatients晰murothelialcarcinomahaveobviouslyincreasedchromosomeabnomilitiescompared麗tllthecontrolgroupAfterthethresholdwcreestabl

6、ished，resultsofFISHandu曲cytologyworecomparedThesensitivityandspecificityofurinecytologywcre71％and80％respectivelythesensitivityandspecificityofFISHwcre88％and80％respectivelythesensitivityofFISHishigherthanurinecytologyAsfo

7、rthelowgradeurothelialcarcinomathesensitivityofurinecytologyandFISHwere49％and78％respectivelyAndforhighgradeurothelialcarcinomathesensitivityofurinecytologyandFISHWel“e88％and96％respectivelyThereceiveroperatingcharacterist