缺氧誘導因子-1α和誘導型一氧化氮合酶在胃癌中的表達研究.pdf

1、學校代碼：10132學員編號：2003107中圖分類號：R7352喲蒙爸逢穹皖i鞘瓣鞋輟MONGO軋l矗黼EDIC焱歉e0乳k蕤GE碩士學位論文缺氧誘導因子一1儀和誘導型一氧化氮合酶在胃癌中的表達研究學科專業(yè)：研究生：指導教師：完成時閥：病理學與瘸理生理學袁宏偉冀文茹教授李超美高級實驗師二0o六年五月oABSTRACllo嗡ectiveHypo)(iaisac(Ⅱ砌onphe㈣enoninthetⅢnornlicroenviroment

2、ofm。stsolidtumourS，includinghurnangastriccancerH)幣0xia—induciblefactor一1a(HIF～1口)isuniverSa王重yexDress耐inmany啪es《越ls，andplaysakeyfolein刪難ationoft眥ourrc—latedgeneSunderhypoxiaoLlrresearchwaStodetectmRNAandp∞teinexpreSsi孤of

3、HIF—handiN()S(induciblenⅫc0xide∞mth矗Se)，andexploreitsrelatiorIshipwithbblogicalcharacteristics《gastriccancer，suchaStumourangiog∞esis，nm跏rdifferentiation，andnletastaSisM“hodsHurnangastriccancercel王SGC一7901wasculturedunder

4、hypoxic。Dnditions(1％oXygen)forlh，6h，12h，24handnoHnoX沁吲1djtions，R肼ersetranScription一耐)硼erasechainre8ction(RT—PCR)andimmunocytocherniStrywereusedtodetectmRNA1evelandproteinlevelexprcssk咀ofHIF—lGandiNoSImmunohistoch鋤istry啪s

5、usedtodetecttheexpressionofHIF～1a，iNoSin45resectedspeciHlenSofgastriccancer8nd10paracarlceroustissueS鼗器ults(1)Semiquantityf汀一PcRs址昭estedthatthee)【一pressionofHIF—lQmRNAinthehypo越cgD叫ps㈣paredwiththenomoxicgroupallhadnotasi

6、gflificantdifference(p005)InnoHnoxicconditiontheexpre蹣knofiNoSn1RNA、^，asataverylowlevelAt1hafterhypo五a，theeXpressionof主NOSmRNAhadnotasig—nificantdifference(pO05)Whi王e6h，12hand24hafterhypoxiatbelevelofexpressionincreasedr

7、apid王y(2)Immunocytocher工1istrysuggestedthatinthenormoxicconditi。ntheexpresShofprotein《HIF—l飪waSn。tdetectedWhiletlep眥einofiNOSwasataverylowlevel。Butbothw黜incrcasingatdifferenthy辯ict婦(3)弧eexpres8ionofHIF一1aandiNOSpmteinwer

8、e667％(30／45)，756％(34／45)ingastriccancerTheexpres—sion《HfF—laproteinweresignificantlycorrelatedwithdifferencia矗on，infil掘牡ion，metaStaSisandTNM(pO。05)Whiletheexpre娥onofiNoSprotein愀s塘Hificantlycor—relatedwithinfiltration，met